Viral wnvNS3 Protease Antibody

Name: Viral wnvNS3 Protease Antibody
Brand: R&D Systems
SKU: AF2907

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2907

Citations (6)

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Key Product Details

Species Reactivity:	Viral
Immunogen:	E. coli-derived recombinant wnvNS3 Protease, expressed as a fusion protein with cofactor NS2L Gly1506-Leu1689 Accession # ABD85079
Applications:	Immunoprecipitation, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects West Nile Virus NS3 Protease in direct ELISAs and Western blots.

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Applications for Viral wnvNS3 Protease Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Viral wnvNS3 Protease (Catalog # 2907‑SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Viral wnvNS3 Protease Antibody

	Western Blot Detection of wnvNS3 Protease by Western Blot. Western blot shows Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Viral wnvNS3 Protease Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. The purified recombinant protein has autocatalytic activity that can lead to protein degradation. It is therefore important to store the sample below -20° C and to keep on ice while working with the sample.

Item	Value
References	Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.
Long Name	West Nile Virus NS3 Protease
Entrez Gene IDs	912267 (Viral)
Alternate Names	wnvNS3 Protease

Citations for Viral wnvNS3 Protease Antibody (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Viral wnvNS3 Protease Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

AI Chiramel et al. (2019), TRIM5? Restricts Flavivirus Replication by Targeting the Viral Protease for Proteasomal Degradation Cell Rep, 27(11):3269-3283.e6.
PMID: 31189110
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

LD Aarreberg et al. (2018), Interleukin-1? Signaling in Dendritic Cells Induces Antiviral Interferon Responses MBio, 9(2).
PMID: 29559569
Species: Hamster
Sample Types: Cell Lysates
Applications: Western Blot

M Basu et al. (2017), Arsenite-induced stress granule formation is inhibited by elevated levels of reduced glutathione in West Nile virus-infected cells PLoS Pathog, 13(2):e1006240.
PMID: 28241074
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Lazear H et al. (2013), IRF-3, IRF-5, and IRF-7 coordinately regulate the type I IFN response in myeloid dendritic cells downstream of MAVS signaling. PLoS Pathog, 9(1):e1003118.
PMID: 23300459
Species: Virus
Sample Types: Cell Lysates
Applications: Western Blot

(2012), Identification of novel host cell binding partners of Oas1b, the protein conferring resistance to flavivirus-induced disease in mice. Oncogene, 86(15):7953-63.
PMID: 22623793
Species: Virus
Sample Types: Cell Lysates
Applications: Western Blot

Wang P et al. (2010), Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I. Nat. Immunol., 11(10):912-9.
PMID: 20818395

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Showing 1 - 6 of 6 citations Showing All

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