West Nile Virus NS3 Protease Biotinylated Antibody

Name: West Nile Virus NS3 Protease Biotinylated Antibody
Brand: R&D Systems
SKU: BAF2907
Price: 483 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF2907

Citations (2)

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Key Product Details

Species Reactivity:	Viral
Immunogen:	E. coli-derived recombinant wnvNS3 Protease, expressed as a fusion protein with cofactor NS2L Gly1506-Leu1689 Accession # ABD85079
Applications:	Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects Viral wnvNS3 Protease in direct ELISAs and Western blots.

View Full Technical Details for West Nile Virus NS3 Protease Biotinylated Antibody

Applications for West Nile Virus NS3 Protease Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2. The purified recombinant protein has autocatalytic activity that can lead to protein degradation. It is therefore important to store the sample below -20° C and to keep on ice while working with the sample.

Item	Value
Long Name	West Nile Virus NS3 Protease
Entrez Gene IDs	912267 (Viral)
Alternate Names	wnvNS3 Protease

Citations for West Nile Virus NS3 Protease Biotinylated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for West Nile Virus NS3 Protease Biotinylated Antibody include:

Species: Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry

MA O'Connor et al. (2018), Early cellular innate immune responses drive Zika viral persistence and tissue tropism in pigtail macaques Nat Commun, 9(1):3371.
PMID: 30135445
Species: Virus
Sample Types: Cell Lysates
Applications: Western Blot

(2012), Infectious clones of novel lineage 1 and lineage 2 West Nile virus strains WNV-TX02 and WNV-Madagascar. J. Virol., 86(14):7704-9.
PMID: 22573862

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West Nile Virus NS3 Protease Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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