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Key Product Details

Species Reactivity

Multi-Species

Applications

Binding Inhibition, ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1D11

Product Summary for TGF-beta 1, 2, 3 Antibody

Immunogen

Bovine bone-derived TGF-beta 1 and TGF-beta 2

Specificity

Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3. No cross-reactivity was observed with recombinant LAP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TGF-beta 1, 2, 3 Antibody

TGF-beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF-beta 1, 2, 3 Antibody.

TGF-beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF-beta 1, 2, 3 Antibody.

Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835). The ND50 is typically 0.25-1.25 µg/mL.
Detection of Mouse TGF beta 1/2/3 by In vivo assay

Detection of Mouse TGF beta 1/2/3 by In vivo assay

Apoptotic cell-induced immunomodulation in mice with collagen-induced arthritis (CIA) is dependent on transforming growth factor (TGF)-beta. CIA mice that received or did not receive apoptotic cells, with or without anti-TGF-beta blocking antibody were scored daily (a). Data are shown as mean ± SEM of five mice per group from one of two representative experiments; *p < 0.05, **p < 0.01 (Friedman test analysis of variance (ANOVA) with Dunn's multiple comparisons test). Anti-collagen IgG2a antibodies (Ab) were quantified in plasma (b). Data from 5 to 16 mice from two to three independent experiments (no difference, Kruskal-Wallis test ANOVA with Dunn’s multiple comparisons test). Lymph node cells were collected, cultured, and T cell proliferation in response to collagen protein or CD3-specific Ab stimulations was evaluated (c). Data are shown as mean ± SEM from one of two representative experiments; *p < 0.05, **p < 0.01 (nonparametric unpaired t test). Percent and absolute number of Foxp3+ T regulatory cells (Treg) were evaluated in the spleen (d) and suppressive assays were performed by isolating and adding Treg at a different ratio into collagen-specific cultures and responder T cell proliferation was assessed (e). Data are shown as mean ± SEM from one experiment (d Kruskal-Wallis test ANOVA with Dunn’s multiple comparisons test; e Friedman test ANOVA with Dunn’s multiple comparisons test). Plasmacytoid dendritic cells (pDC), conventional dendritic cells (cDC) and macrophages (macro) were isolated and cultured with naïve allogenic T cells to determine Treg polarization (f), and their response to TLR ligand was assessed through evaluation of CD40 costimulatory molecule expression (g). Data from individual mouse plus mean (black bar) from one experiment representative of four are shown; *p < 0.05, ***p < 0.001 (f ordinary one-way ANOVA with Tukey’s multiple comparisons test; g Kruskal-Wallis test ANOVA with Dunn’s multiple comparisons test). BrDU 5-Bromo-2’-deoxyuridine Image collected and cropped by CiteAb from the following publication (http://arthritis-research.biomedcentral.com/articles/10.1186/s13075-016-1084-0), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human TGF beta 1/2/3 by Immunocytochemistry/Immunofluorescence

Detection of Human TGF beta 1/2/3 by Immunocytochemistry/Immunofluorescence

TGF beta1 stimulates the expression of ECM components in human and rabbit primary cells. (A) Selected ECM mRNA transcripts were measured using qRT-PCR in human RPTEC or human IPF134 following 48 hr stimulation with TGF beta1 (10 ng/mL). Data show transcript abundance relative to the unstimulated control (indicated by the dashed line) and are shown as mean ± SD of four (RPTEC) or seven (IPF134) independent experiments. ****P < 0.0001; ****P < 0.001; **P < 0.01; Student’s t-test. (B) The accumulation of ECM in human RPTEC (i) or human IPF (ii) cells stimulated with 10ng/mL TGF beta1 was evaluated via measurement of the incorporation of 14C-labelled amino acids into the deposited ECM. Data presented are the mean ± SD of three independent experiments. **P < 0.01; Student’s t-test. (C) Increased mature ECM accumulation following treatment of cells with a pro-fibrotic stimulus can be visualised following in-situ fluorescent staining using the Flamingo dye. Cells from three different systems (primary human RPTEC; primary human IPF134; co-culture of primary rabbit RPTEC with primary rabbit renal fibroblasts) were cultured in the absence (top) or presence (bottom) of 10 ng/ml TGF beta1 before decellularised matrix was fixed and stained in situ with Flamingo fluorescent dye. Images show a single field. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28855577), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for TGF-beta 1, 2, 3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta 1 (Catalog # 240-B) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize TGF‑ beta1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1990) Lymphokine Res. 9:607. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 1 ng/mL Recombinant Human TGF‑ beta1 and 7.5 ng/mL Recombinant Mouse IL‑4.

Binding Inhibition

Dasch, J.R. et al. (1994) J. Immunol. 142:1536.

Human TGF-beta 1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: TGF-beta 1 Biotinylated Antibody (Catalog # BAF240)
  • Standard: Recombinant Human TGF-beta 1 Protein (Catalog # 240-B)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 12 reviews rated 4.4 using MAB1835 in the following applications:

Published Applications

Read 215 publications using MAB1835 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1, 2, 3

TGF-beta 1, -2, and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.

References

  1. Ayala A. et al. (1992) FASEB J. 6:A1604.
  2. Roberts A.B. and Sporn M.B., eds. (1990) Peptide Growth Factors and Their Receptors I, Springer-Verlag, 419.
  3. Dasch J.R. et al. (1989) J. Immunol. 142:1536.

Long Name

Transforming Growth Factor beta 1, 2, and 3

Additional TGF-beta 1, 2, 3 Products

Product Documents for TGF-beta 1, 2, 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for TGF-beta 1, 2, 3 Antibody

For research use only

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TGF-beta 1, 2, 3 Antibody

Showing  1 - 1 of 1 FAQ Showing All
  • Q: Can the TGF-beta 1, 2, 3 Antibody, Cat# MAB1835, neutralize the activity of all three TGF-beta proteins?

    A: Yes, this antibody can neutralize the activity of TGF-beta 1, 2, and 3 proteins.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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