Rat IL-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF-504-NA

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AF-504-SP
AF-504-NA
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IL-4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IL-4
Cys25-Ser147
Accession # P20096

Specificity

Detects rat IL-4 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant cotton rat IL‑4 and recombinant mouse IL-4 is observed, and less than 1% cross-reactivity with recombinant human IL‑4, recombinant canine IL-4, and recombinant porcine IL‑4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat IL-4 Antibody

Cell Proliferation Induced by IL‑4 and Neutralization by Rat IL‑4 Antibody.

Cell Proliferation Induced by IL‑4 and Neutralization by Rat IL‑4 Antibody.

Recombinant Rat IL-4 (Catalog # 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-4 (4 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA). The ND50 is typically 0.05-015 µg/mL in the presence of PHA (10 µg/mL).
Detection of IL-4 by Western Blot

Detection of IL-4 by Western Blot

HFSCs and HFSCs/HGF suppressed inflammatory reaction. A–E Iba-1 staining in the penumbra at 7 days after cell transplantation. Scale bar = 100 µm. F The quantitative analysis of the numbers of Iba-1-positive cells. G Representative western blot bands of inflammation-related proteins in different groups. H and I Quantification of TNF-a and IL-4 expression in the ischemic hemisphere extracts. Values are the mean ± SD. *P < 0.05 Saline group vs. Control group, #P < 0.05 HFSCs group vs. Saline group, ∆P < 0.05 HFSCs/HGF group vs. HFSCs/Vehicle group, n = 6 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36782269), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-4 by Western Blot

Detection of IL-4 by Western Blot

HFSCs and HFSCs/HGF suppressed inflammatory reaction. A–E Iba-1 staining in the penumbra at 7 days after cell transplantation. Scale bar = 100 µm. F The quantitative analysis of the numbers of Iba-1-positive cells. G Representative western blot bands of inflammation-related proteins in different groups. H and I Quantification of TNF-a and IL-4 expression in the ischemic hemisphere extracts. Values are the mean ± SD. *P < 0.05 Saline group vs. Control group, #P < 0.05 HFSCs group vs. Saline group, ∆P < 0.05 HFSCs/HGF group vs. HFSCs/Vehicle group, n = 6 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36782269), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Applications for Rat IL-4 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat IL-4 (Catalog # 504-RL)

Neutralization

Measured by its ability to neutralize IL-4-induced proliferation in the rat splenocytes. The Neutralization Dose (ND50) is typically 0.05-015 µg/mL in the presence of 4 ng/mL Recombinant Rat IL-4 and 10 µg/mL PHA.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure (4). Rat IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

References

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. McKnight, A.J. et al. (1991) Eur. J. Immunol. 21:1187.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 751514 (Feline); 574281 (Primate); 100302454 (Rabbit)

Gene Symbol

IL4

UniProt

P20096

Additional IL-4 Products

Product Documents for Rat IL-4 Antibody

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Product Specific Notices for Rat IL-4 Antibody

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