Recombinant Rat IL-4 Protein

Name: Recombinant Rat IL-4 Protein
Brand: R&D Systems
SKU: 504-RL
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 504-RL

Citations (13)

(2)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	P20096.2
Applications:	Bioactivity

View Full Technical Details for Recombinant Rat IL-4 Protein

Product Specifications for Recombinant Rat IL-4 Protein

Source	E. coli-derived rat IL-4 protein Cys25-Ser147, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	13.5 kDa
Activity	Measured in a cell proliferation assay using rat splenocytes. Noble, A. and D.M. Kemery (1995) Immunology 85:357. The ED₅₀ for this effect is 0.025-0.25 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

504-RL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

504-RL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rat IL-4 is synthesized with a 24 aa signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5 ‑ 7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL‑13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

Item	Value
References	Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1. McKnight, A.J. et al. (1991) Eur. J. Immunol. 21:1187. Redfield, C. et al. (1991) Biochemistry 30:11029. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451. Mosman, T.R. et al. (1987) J. Immunol. 138:1813. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701. Paludan, S.R. (1998) Scand. J. Immunol. 48:459. Corthay, A. (2006) Scand. J. Immunol. 64:93. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name	Interleukin 4
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names	B cell growth factor 1, B-cell stimulatory factor 1, BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL-4B_cell stimulatory factor 1, IL4, IL4E12, Lymphocyte stimulatory factor 1, MGC79402, binetrakin, interleukin 4, interleukin-4, pitrakinra

Citations for Recombinant Rat IL-4 Protein (13)

Showing 1 - 10 of 13 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat IL-4 Protein include:

Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

S Kim et al. (2021), Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia International Journal of Molecular Sciences, 22(16).
PMID: 34445510
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

IKA Jones et al. (2020), Rat Cytomegalovirus Virion-Associated Proteins R131 and R129 Are Necessary for Infection of Macrophages and Dendritic Cells Pathogens, 9(11).
PMID: 33228102
Species: Rat
Sample Types: Tissue Explant
Applications: Bioassay

CJ Lawrimore et al. (2019), Ethanol Induction of Innate Immune Signals Across BV2 Microglia and SH-SY5Y Neuroblastoma Involves Induction of IL-4 and IL-13 Brain Sci, 9(9).
PMID: 31510019
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

S Lively et al. (2018), Microglia Responses to Pro-inflammatory Stimuli (LPS, IFN?+TNF?) and Reprogramming by Resolving Cytokines (IL-4, IL-10) Front Cell Neurosci, 12(0):215.
PMID: 30087595
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

SU Vay et al. (2018), The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo J Neuroinflammation, 15(1):226.
PMID: 30103769
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

HT Lee et al. (2017), A Crucial Role of CXCL14 for Promoting Regulatory T Cells Activation in Stroke Theranostics, 7(4):855-875.
PMID: 28382159
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

JE Lim et al. (2017), A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN? Sci Rep, 7(1):9417.
PMID: 28842601
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

T Ito et al. (2017), Secreted Ectodomain of SIGLEC-9 and MCP-1 Synergistically Improve Acute Liver Failure in Rats by Altering Macrophage Polarity Sci Rep, 7(0):44043.
PMID: 28272428
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

Lim S et al. (2013), Neuronal synapse formation induced by microglia and interleukin 10. PLoS ONE, 8(11):e81218.
PMID: 24278397
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Krejci P et al. (2009), Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes. Cell. Signal., 21(1):151-60.
PMID: 18950705
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Butovsky O et al. (2006), Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis. J. Clin. Invest., 116(4):905-15.
PMID: 16557302
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Azhipa O et al. (2005), Comparative analysis of the fate of donor dendritic cells and B cells and their influence on alloreactive T cell responses under tacrolimus immunosuppression. Clin. Immunol., 114(2):199-209.
PMID: 15639654
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Siddiqui T et al. (0), Expression and contributions of TRPM7 and KCa2.3/SK3 channels to the increased migration and invasion of microglia in anti-inflammatory activation states. PLoS ON9(8):e106087.
PMID: 25148577

There are no citations that match your criteria.

Showing 1 - 10 of 13 citations Showing All

Customer Reviews for Recombinant Rat IL-4 Protein (2)

5 out of 5

2 customer ratings

5 stars

100%

4 stars

3 stars

2 stars

1 star

Showing 1 - 2 of 2 reviews Showing All

Filter By:

Recombinant Rat IL-4 Protein

Name: Anonymous

Application: CellProlif

Reason for Rating: Excellent biological activity as tested by cell proliferation assay.

Verified Customer | Posted 11/20/2021
Recombinant Rat IL-4 Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 08/22/2016

Recombinant Rat IL-4 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Recombinant Rat IL-4 Protein

Formulation, Preparation and Storage

Carrier Free

504-RL

Carrier

504-RL/CF

Carrier Free

Reconstitution Calculator

Background: IL-4

Citations for Recombinant Rat IL-4 Protein (13)

Customer Reviews for Recombinant Rat IL-4 Protein (2)

Recombinant Rat IL-4 Protein

Recombinant Rat IL-4 Protein

Product Documents for Recombinant Rat IL-4 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected