Detects rat IL-1 beta in direct ELISAs and Western blots. In these formats, this antibody shows approximately 100% cross‑reactivity with recombinant mouse (rm) IL-1 beta and rcrIL-1 beta and no cross-reactivity with rfIL‑1 beta, rhIL-1 beta, or rrmIL-1 beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Rat IL-1 beta/IL-1F2 Antibody

Detection of Rat IL-1 beta/IL-1F2 by Western Blot

Plantaris protein content results for interleukin-1 beta (IL-1 beta) (A). Data were normalized to CON. Values are mean ± SEM (CON: n = 4; SNL: n = 4; 100CUR: n = 4; 50CMO: n = 4). Φ p < 0.050 vs. ipsilateral leg. The representative blot images display IL-1 beta and alpha-tubulin for CON, SNL, 100CUR, and 50CMO groups (B). MW, molecular weight (kDa). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1467-3045/46/11/742), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Rat IL-1 beta/IL-1F2 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat IL-1 beta/IL-1F2 (Catalog # 501-RL)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

Q63264

Additional IL-1 beta/IL-1F2 Products

Product Documents for Rat IL-1 beta/IL-1F2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat IL-1 beta/IL-1F2 Antibody

For research use only

Citations
Reviews

Rat IL-1 beta/IL-1F2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5011

Key Product Details

Species Reactivity

Validated:

Cited:

Applications