Rat 4-1BB/TNFRSF9/CD137 Alexa Fluor™ Plus 555-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF9029AFP555

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AF9029AFP555-100UG
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Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Polyclonal Goat IgG

View all 4-1BB/TNFRSF9/CD137 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat 4-1BB.

Specificity

Detects rat 4-1BB in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: 4-1BB/TNFRSF9/CD137

4‑1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4‑1BB functions in the development and activation of multiple immune cells (1). Mature rat consists of a 166 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain. Within the ECD, rat 4‑1BB shares 60% and 79% aa sequence identity with human and mouse 4‑1BB, respectively. 4‑1BB is expressed as a disulfide‑linked homodimer on various populations of activated T cells including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells (2‑5) as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts (6‑9). It binds with high affinity to the transmembrane 4‑1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells (6, 10). This interaction co‑stimulates the proliferation, activation, and/or survival of the 4‑1BB expressing cell (2‑5, 10). It can also enhance the activation‑induced cell death of repetitively stimulated T cells (10). Mice lacking 4‑1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells (11). 4‑1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation (12). Reverse signaling through 4‑1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts (6, 9) but supports mature dendritic cell survival and co‑stimulates the proliferation and activation of mast cells (7, 8). 4‑1BB activation enhances CD8+ T cell and NK cell mediated anti‑tumor immunity (13). It also contributes to the development of inflammation in high fat diet‑induced metabolic syndrome (14). Soluble forms of 4‑1BB and 4‑1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively (15, 16).

References

  1. Wang, C. et al. (2009) Immunol. Rev. 229:192.
  2. Wen, T. et al. (2002) J. Immunol. 168:4897.
  3. Pulle, G. et al. (2006) J. Immunol. 176:2739.
  4. Zheng, G. et al. (2004) J. Immunol. 173:2428.
  5. Kim, D. et al. (2008) J. Immunol. 180:2062.
  6. Lee, S. et al. (2008) Nat. Immunol. 9:917.
  7. Choi, B.K. et al. (2009) J. Immunol. 182:4107.
  8. Nishimoto, H. et al. (2005) Blood 106:4241.
  9. Saito, K. et al. (2004) J. Biol. Chem. 279:13555.
  10. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  11. Lee, S. et al. (2005) J. Immunol. 174:6803.
  12. Ma, B.Y. et al. (2005) Blood 106:2002.
  13. Choi, B.K. et al. (2010) J. Immunol. 185:1404.
  14. Kim, C. et al. (2011) Diabetes 60:3159.
  15. Michel, J. et al. (1998) Eur. J. Immunol. 28:290.
  16. Salih, H.R. et al. (2001) J. Immunol. 167:4059.

Alternate Names

41BB, CD137, ILA, TNFRSF9

Entrez Gene IDs

3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)

Gene Symbol

TNFRSF9

Additional 4-1BB/TNFRSF9/CD137 Products

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Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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