PDGF R beta Antibody (APB5) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43349
![Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]](https://resources.bio-techne.com/images/products/PDGF-R-beta-Antibody-APB5-Flow-Cytometry-NBP1-43349-img0004.jpg "Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Protein Array
Cited:
Immunohistochemistry-Frozen, Flow Cytometry, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # APB5
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was PDGF receptor beta.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for PDGF R beta Antibody (APB5) - BSA Free
Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]
Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Mouse brain pericytes - IgG (left) and PDGFRB (right). Image from verified customer review.![Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]](https://resources.bio-techne.com/images/products/PDGF-R-beta-Antibody-APB5-Flow-Cytometry-NBP1-43349-img0001.jpg "Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]")
Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]
Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (cat. 14-4321) (open histogram) or 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Purified (filled histogram) followed by Anti-Rat IgG Biotin (cat. 13-4813) and Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.![Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]](https://resources.bio-techne.com/images/products/PDGF-R-beta-Antibody-APB5-Flow-Cytometry-NBP1-43349-img0003.jpg "Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]")
Flow Cytometry: PDGF R beta Antibody (APB5) - BSA Free [NBP1-43349]
Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Analysis using the Biotin conjugate of NBP1-43349. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram).Applications for PDGF R beta Antibody (APB5) - BSA Free
Application
Recommended Usage
Flow Cytometry
< 1.0 ug/10^6 cells
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Application Notes
The APB5 antibody has been tested by flow cytometric analysis of NIH-3T3 cells. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Use in Protein Array reported in secitific publication PMID: 32499572
Reviewed Applications
Read 1 review rated 5 using NBP1-43349 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PDGF R beta
Long Name
Platelet-derived Growth Factor Receptor beta
Alternate Names
CD140b, PDGFRB
Entrez Gene IDs
18596 (Mouse)
Gene Symbol
PDGFRB
UniProt
Additional PDGF R beta Products
Product Documents for PDGF R beta Antibody (APB5) - BSA Free
Product Specific Notices for PDGF R beta Antibody (APB5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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