PD-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88104
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: WFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAIS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PD-1 Antibody - BSA Free (NBP1-88104) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-PD-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PD-1 Antibody - BSA Free
Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]
Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104]
Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-88104] - Staining of human liver shows no positivity in hepatocytes as expected.Applications for PD-1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Simple Western
1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: NK cell lysate at 1 mg/ml, NSCLC lysate at 1 mg/ml as samples; separated by size; antibody dilution of 1:50; observed molecular weight was 116 kDa; matrix was 12-230 kDa; detected by Chemiluminescence.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PD-1
Long Name
Programmed Death-1
Alternate Names
CD279, PD1, PDCD1, SLEB2
Gene Symbol
PDCD1
Additional PD-1 Products
Product Documents for PD-1 Antibody - BSA Free
Product Specific Notices for PD-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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