PD-1 Antibody (J43) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43110
![Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]](https://resources.bio-techne.com/images/products/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0007.jpg "Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]")
![Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]](https://resources.bio-techne.com/images/products/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0008.jpg "Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]")
![Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]](https://resources.bio-techne.com/images/products/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0009.jpg "Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Armenian Hamster IgG Clone # J43
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was PD1.
Clonality
Monoclonal
Host
Armenian Hamster
Isotype
IgG
Scientific Data Images for PD-1 Antibody (J43) - BSA Free
Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis of PE conjugate of NBP1-43110. The J43 antibody has been tested by flow cytometric mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be de![Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]](https://resources.bio-techne.com/images/products/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0002.jpg "Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]")
Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster Ig![Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]](https://resources.bio-techne.com/images/products/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0003.jpg "Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]")
Flow Cytometry: PD-1 Antibody (J43) - BSA Free [NBP1-43110]
Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Biotin conjugate of NBP1-43110. Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.Applications for PD-1 Antibody (J43) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.IHC-F reactivity reported in scientific literature (PMID: 12847137 )
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PD-1
Long Name
Programmed Death-1
Alternate Names
CD279, PD1, PDCD1, SLEB2
Gene Symbol
PDCD1
Additional PD-1 Products
Product Documents for PD-1 Antibody (J43) - BSA Free
Product Specific Notices for PD-1 Antibody (J43) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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