Neprilysin/CD10 Antibody - BSA Free

Western Blot: Neprilysin/CD10 Antibody - BSA Free [NBP2-15771] -

MRPL49 protein is a substrate of neprilysin. (A) Representative WB analysis showing the rescue of MRPL49 levels, after neprilysin knock-down (KD). siCTRL: pool of control siRNAs. siNEP: pool of neprilysin-silencing siRNAs. (B) Relative fold-change in the levels of neprilysin protein in both controls (siCTRL) and neprilysin-silenced (siNEP) cells upon DA treatment, showing the effectiveness of the KD. Three biological replicates (n=3). Error bars: SEM. Statistical analysis performed by two-way ANOVA, to assess the effects of both “KD" (siCTRL vs. siNEP) and “treatment" (CTRL vs. DA). The only significant source of variation was “KD" (p = 0.004; F = 15.98). (C) Relative fold-change in the levels of MRPL49 protein in siCTRL and siNEP cells upon DA treatment, showing the rescue of the levels of MRPL49 upon neprilysin KD. Three biological replicates (n=3). Error bars: SEM. Statistical analysis performed by two-way ANOVA. Both “treatment" (p = 0.0057; F = 14.02) and “interaction" (p = 0.040; F = 5.98) resulted to be significant sources of variation. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31417398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Neprilysin/CD10 Antibody - BSA Free [NBP2-15771] -

Neprilysin expression and localization in SH-SY5Y cells. (A) Representative WB analysis showing the presence of neprilysin in SH-SY5Y cells (T: total extracts) and its enrichment in mitochondrial isolates (M). (B) Relative fold-change in the levels of neprilysin in both total (T) and mitochondrial (M) extracts upon DA treatment, showing the significant enrichment of neprilysin in the mitochondrial fraction. Normalization was based on total protein amount per lane. Three biological replicates (n=3). Error bars: SEM. Statistical analysis performed by two-way ANOVA, to assess the effects of both “localization" (T vs. M) and “treatment" (CTRL vs. DA). The only significant source of variation was “localization" (p = 0.0002; F = 40.55). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31417398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.