Mouse YM1/Chitinase 3-like 3 Antibody

Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot.

Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse YM1/Chitinase 3‑like 3 by Simple Western^TM.

Simple Western lane view shows lysates of mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for YM1/Chitinase 3-like 3 at approximately 49 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of YM1/Chitinase 3-like 3 by Western Blot

TRPV1 suppresses the expression of Arg1 and Ym1 in activated microglia. (A,B) Quantitative PCR (qPCR) analysis of Chil3(A) and Arg1(B) mRNA expression in cortical tissues of WT (n = 6) and TRPV1−/−(n = 6) mice after rFS. Values were shown for steady-state transcripts relative to beta-actin. Data were presented as mean ± SEM (n = 6 per groups, *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA followed by Tukey’s multiple comparisons). (C–E) Representative Immunoblot bands (C) and densitometric analysis of Arg1 expression (D) and Ym1 expression (E) were significantly decreased in rFS from WT mice than in TRPV1−/− mice (n = 6 per groups, *p < 0.05, ***p < 0.001, two-way ANOVA followed by Tukey’s multiple comparisons). (F–H) Representative Immunoblot bands (F) and densitometric analysis of Arg1 expression (G) and Ym1 expression (H) was significantly decreased in the cortex microglia of WT mice than in TRPV1−/− mice after treatment of Capsaicin (10 μM), Hyperthermia (43°C, 4*30 min) and LPS (1.0 μg/ml), respectively. Data were presented as means ± SEM, n = 6 per groups, *p < 0.05, **p < 0.01, two-way ANOVA followed by Tukey’s multiple comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31680864), licensed under a CC-BY license. Not internally tested by R&D Systems.