Mouse TROP-2 Antibody

Detection of Mouse TROP-2 by Immunohistochemistry

Gene expression analysis of Pten mutant prostates.(A) i) Heatmap demonstrating that control samples (C1-C5) show similar expression patterns to each other, as do mutant samples (M1-M5). ii) Genes chosen for validation are indicated in heatmap derived from the average of control and mutant samples. Red indicates genes that are upregulated in mutants (116 genes), relative to controls, and green indicates genes that are downregulated in the mutants relative to controls (91 genes). (B) Quantitative RTPCR validation of indicated androgen-dependent genes differentially expressed in mutants and controls (Clu p = 0.001, Trop2 p = 0.002, Nkx3.1 p = 0.02, and Pbsn p = 0.003). mRNA accumulation was normalized to GAPDH. (C) Differential antibody staining of CLU, TROP2, and PBSN at P10 validated microarray results. (D) Whole mount in situ hybridization of Nkx3.1 shows a decrease in expression in P10 mutant prostates. The anterior prostate (AP), dorsal lateral prostate (DL) and urethra (UR) are indicated. Red arrows indicate presence of stain in the controls and absence in the mutant. “*” denotes statistical significance. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0129470), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse TROP-2 by Immunohistochemistry-Paraffin

Pten deletion does not lead to an accumulation of progenitor cells.Antibody staining on sections of P0 and P7 prostates with antibodies to CLU and TROP2 shows no accumulation of these proteins at earlier stages of development in controls (A, B, C, D). Increased expression of these markers is seen in the mutant (E, F, G, H) relative to controls from P7. Red arrowheads indicate buds. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0129470), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse TROP-2 by Immunohistochemistry

Gene expression analysis of Pten mutant prostates.(A) i) Heatmap demonstrating that control samples (C1-C5) show similar expression patterns to each other, as do mutant samples (M1-M5). ii) Genes chosen for validation are indicated in heatmap derived from the average of control and mutant samples. Red indicates genes that are upregulated in mutants (116 genes), relative to controls, and green indicates genes that are downregulated in the mutants relative to controls (91 genes). (B) Quantitative RTPCR validation of indicated androgen-dependent genes differentially expressed in mutants and controls (Clu p = 0.001, Trop2 p = 0.002, Nkx3.1 p = 0.02, and Pbsn p = 0.003). mRNA accumulation was normalized to GAPDH. (C) Differential antibody staining of CLU, TROP2, and PBSN at P10 validated microarray results. (D) Whole mount in situ hybridization of Nkx3.1 shows a decrease in expression in P10 mutant prostates. The anterior prostate (AP), dorsal lateral prostate (DL) and urethra (UR) are indicated. Red arrows indicate presence of stain in the controls and absence in the mutant. “*” denotes statistical significance. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0129470), licensed under a CC-BY license. Not internally tested by R&D Systems.