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Mouse TRANCE/TNFSF11/RANK L Antibody

Catalog # AF462 | R&D Systems, Inc. a Bio-Techne Brand
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AF462
AF462-SP

Key Product Details

Species Reactivity

Mouse

Applications

ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse TRANCE/TNFSF11/RANK L Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TRANCE/TNFSF11/RANK L
Arg72-Asp316
Accession # O35235

Specificity

Detects mouse TRANCE/TNFSF11/RANK L in ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TRANCE/TNFSF11/RANK L Antibody

Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody.

Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody.

In the presence of Recombinant Mouse M-CSF (20 ng/mL, 416-ML), Recombinant Mouse TRANCE/TNFSF11/RANK L (462-TR) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF11/ RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF11/RANK L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF462). The ND50 is typically 0.02-0.06 µg/mL.

Applications for Mouse TRANCE/TNFSF11/RANK L Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR)

Neutralization

Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in mouse splenocytes. Nakagawa, N. et al. (1998) Biochim. Biophys. Res. Commun. 253:395. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 30 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L.

Mouse TRANCE/TNFSF11/RANK L Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody (Catalog # BAF462)
  • Standard: Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein (Catalog # 462-TR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using AF462 in the following applications:

Published Applications

Read 25 publications using AF462 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

References

  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

UniProt

Product Documents for Mouse TRANCE/TNFSF11/RANK L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TRANCE/TNFSF11/RANK L Antibody

For research use only

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