Mouse Sonic Hedgehog/Shh N-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4641
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Neutralization, ELISA Capture, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 171018
Product Specifications
Immunogen
E. coli-derived recombinant mouse Sonic Hedgehog
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Specificity
Detects mouse Sonic Hedgehog in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Dhh(N‑terminus; aa 23‑198) is observed and no cross-reactivity with rmIhh (N-terminus; aa 66‑240), rmDhh (C‑terminus; aa 199‑396), or rmShh (C‑terminus; aa 199-437) is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse Sonic Hedgehog/Shh N-Terminus Antibody
Alkaline Phosphatase Production Induced by Sonic Hedgehog and Neutralization by Mouse Sonic Hedgehog Antibody.
Recombinant Mouse Sonic Hedgehog N-Terminus (Catalog # 461-SH or 464-SH) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse Sonic Hedgehog N-Terminus (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Sonic Hedgehog N-Terminus Monoclonal Antibody (Catalog # MAB4641). The ND50 is typically 5-15 µg/mL.
Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot.
Western blot shows lysates of human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Sonic Hedgehog/Shh in Mouse Embryo.
Sonic Hedgehog/Shh was detected in immersion fixed frozen sections of mouse embryo (11 d.p.c.) using Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Sonic Hedgehog/Shh N-Terminus Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain and immersion fixed frozen sections of mouse embryo (11 d.p.c.)
Sample: Perfusion fixed frozen sections of mouse brain and immersion fixed frozen sections of mouse embryo (11 d.p.c.)
Western Blot
2 µg/mL
Sample: Human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue
Sample: Human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue
Neutralization
Measured by its ability to neutralize Sonic Hedgehog-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 5 µg/mL Recombinant Mouse Sonic Hedgehog N-Terminus.
Mouse Sonic Hedgehog Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sonic Hedgehog/Shh
Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.
Alternate Names
HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS
Gene Symbol
SHH
UniProt
Additional Sonic Hedgehog/Shh Products
Product Documents for Mouse Sonic Hedgehog/Shh N-Terminus Antibody
Product Specific Notices for Mouse Sonic Hedgehog/Shh N-Terminus Antibody
For research use only
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