Mouse Serum Amyloid A1/A2 Antibody

Detection of Mouse Serum Amyloid A1/A2 by Simple Western^TM.

Simple Western lane view shows mouse serum, loaded at a 1:100 dilution. A specific band was detected for Serum Amyloid A1/A2 at approximately 14 kDa (as indicated) using 50 µg/mL of (Catalog # AF2948) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Mouse Serum Amyloid A1/A2 ELISA Standard Curve.

Recombinant Mouse Serum Amyloid A1/A2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Detection of Serum Amyloid A1/A2 by Western Blot

Robust expression of acute phase response proteins in skeletal muscle versus liver in C26 cachexia.A. Western blotting and quantitation of fibrinogen levels in control and C26 quadriceps and liver. Data (mean ± SEM) are expressed as relative densitometry value. **P<0.01, ***P<0.001. B, Western blotting analysis of fibrinogen standard proteins and quadriceps and liver extracts for control, CHO-IL6 injected nude mice and C26 injected CD2F1 mice. Quantitation was performed on the band indicated by the arrow. Data (means ± SEM) are expressed as ng fibrinogen / µg protein. *P<0.05, **P<0.01, ***P<0.001. C, Western blotting analysis demonstrates significantly increased fibrinogen and SAA1 protein levels in quadriceps and gastrocnemius in moderate and severe C26 cachexia. *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21799891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Serum Amyloid A1/A2 by Western Blot

FMT-AD neither induced peripheral inflammation nor caused intestinal damage to recipient mice. (a) Western blot showing protein Serum Amyloid A (SAA) levels in the serum from FMT-AD and FMT-young (FMT-y) mice at 3 d post-TBI. (b) Quantification of the western blot shows a not statistically significant increase in SAA in the serum of males after FMT-AD compared to FMT-y. (c) Representative histology images from intestinal tissue do not show any abnormality between the FMT-y and FMT-AD groups in male or female mice. Left, Hematoxylin and eosin (H.E.) staining; right (×10 Objective), Alcian blue staining (×20 Objective). (d) Densiometric quantification of the Alcian blue positive area and counting the number of goblet cells per villus are not different between FMT-y and FMT-AD groups. Male and female data were combined, since two-way ANOVA showed no sex differences (Alcian blue area, p = 0.429; goblet cells, p = 0.471). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35562867), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Serum Amyloid A1/A2 by Western Blot

FMT-AD neither induced peripheral inflammation nor caused intestinal damage to recipient mice. (a) Western blot showing protein Serum Amyloid A (SAA) levels in the serum from FMT-AD and FMT-young (FMT-y) mice at 3 d post-TBI. (b) Quantification of the western blot shows a not statistically significant increase in SAA in the serum of males after FMT-AD compared to FMT-y. (c) Representative histology images from intestinal tissue do not show any abnormality between the FMT-y and FMT-AD groups in male or female mice. Left, Hematoxylin and eosin (H.E.) staining; right (×10 Objective), Alcian blue staining (×20 Objective). (d) Densiometric quantification of the Alcian blue positive area and counting the number of goblet cells per villus are not different between FMT-y and FMT-AD groups. Male and female data were combined, since two-way ANOVA showed no sex differences (Alcian blue area, p = 0.429; goblet cells, p = 0.471). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35562867), licensed under a CC-BY license. Not internally tested by R&D Systems.