Recombinant Mouse Serum Amyloid A1/A2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse Serum Amyloid A1/A2 Monoclonal Antibody (Catalog # MAB2948) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Mouse Serum Amyloid A1/A2 Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Mouse Serum Amyloid A1/A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2948).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Serum Amyloid A DuoSet ELISA Kit (Catalog # DY2948-05) for convenient development of a sandwich ELISA or the Mouse Serum Amyloid A Quantikine ELISA Kit (Catalog # MSAA00) for a complete optimized ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A1/A2

Mouse Serum Amyloid A Protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated polypeptide and circulates as part of the HDL complex. The SAA1 gene is one of three SAA genes in mouse, and, based on human, is likely to be allelic. The SAA1 gene product differs from the SAA2 gene product by only nine amino acids. In human, circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one to three amino acids being cleaved from either the N- or C-terminus. The same situation may exist in mouse. The amino acid sequence of mature mouse SAA1 is 72%, 72%, 67%, and 74% identical to mature human, rabbit, equine, and hamster SAA1, respectively.

Alternate Names

PIG4;SAA;SAA1;SAA2;Serum amyloid A-1 protein;Serum amyloid A-2 protein;TP53I4

UniProt

P05366

Additional Serum Amyloid A1/A2 Products

Product Documents for Mouse Serum Amyloid A1/A2 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Serum Amyloid A1/A2 Antibody

For research use only

Citations
Reviews

Mouse Serum Amyloid A1/A2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2948

Key Product Details

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Product Specifications

Immunogen

Specificity

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Isotype

Scientific Data Images for Mouse Serum Amyloid A1/A2 Antibody

Mouse Serum Amyloid A1/A2 ELISA Standard Curve.