Detects mouse Semaphorin 4D in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Semaphorin 3F is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse Semaphorin 4D/CD100 Antibody

Detection of Mouse Semaphorin 4D/CD100 by Western Blot.

Western blot shows lysates of mouse brain and thymus tissue under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Rat Anti-Mouse Semaphorin 4D/CD100 Monoclonal Antibody (Catalog # MAB5235) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for Semaphorin 4D/CD100 was detected at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Semaphorin 4D/CD100 Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: mouse brain and thymus tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4D/CD100

Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Semaphorin 4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Semaphorin 4D shares 87% and 93% aa identity with human and rat Semaphorin 4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Semaphorin 4D binds Plexin B1, but also CD72 on B and dendritic cells. In developing neurons, Semaphorin 4D assists in guidance. Semaphorin 4D is proangiogenic when expressed by tumor-associated macrophages.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Entrez Gene IDs

10507 (Human); 20354 (Mouse); 306790 (Rat)

Gene Symbol

SEMA4D

UniProt

NP_038688

Additional Semaphorin 4D/CD100 Products

Product Documents for Mouse Semaphorin 4D/CD100 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Semaphorin 4D/CD100 Antibody

For research use only

Citations
Reviews

Mouse Semaphorin 4D/CD100 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5235

Key Product Details

Species Reactivity

Validated:

Cited:

Applications