Mouse S100A8 Antibody

Name: Mouse S100A8 Antibody
Brand: R&D Systems
SKU: MAB3059
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB3059

Citations (6)

(1)

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Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse S100A8 Met1-Glu89 (predicted) Accession # P27005
Applications:	Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2B Clone # 335806
Specificity:	Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 40% cross-reactivity with recombinant human (rh) S100A8 is observed and no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A9, rmS100A10, rmS100A11, rhS100B, or rhS100P is observed.

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Applications for Mouse S100A8 Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse S100A8 Antibody

	Western Blot Detection of S100A8 Rat by Western Blot. Western blot shows lysates of mouse leukocyte. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry S100A8 in NIH-3T3 Mouse Cell Line. S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10 µg/mL Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: S100A8

Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10 kDa member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal alpha -helix, followed by a calcium-binding EF-hand segment, and a C-terminal alpha -helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 shares 80% and 57% aa sequence identity with rat and human S100A8, respectively.

Item	Value
Long Name	S100 Calcium Binding Protein A8
Entrez Gene IDs	6279 (Human); 20201 (Mouse)
Alternate Names	60B8AG, CAGACP-10, CFAG, CFAGL1Ag, CGLA, Calgranulin A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MA387, MIF, MRP-8, MRP8, MRP8S100 calcium binding protein A8 (calgranulin A), Migration inhibitory factor-related protein 8, NIF, P8, S100 calcium binding protein A8, S100 calcium-binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100A8, Urinary stone protein band A, calgranulin-A, protein S100-A8

Citations for Mouse S100A8 Antibody (6)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse S100A8 Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: ICC

VC Sinha et al. (2021), Single-cell evaluation reveals shifts in the tumor-immune niches that shape and maintain aggressive lesions in the breast Nature Communications, 12(1):5024.
PMID: 34408137
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

N Makthal et al. (2017), A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development EBioMedicine, 0(0).
PMID: 28596134
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Dessing et al. (2015), The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion. Kidney Int, 87(1):85-94.
PMID: 24940802
Species: Mouse
Sample Types: Whole Tissue
Applications: in vitro

Sekimoto R et al. (2015), Visualized macrophage dynamics and significance of S100A8 in obese fat. Proc Natl Acad Sci U S A, 112(16):E2058-66.
PMID: 25848057
Species: Mouse
Sample Types: Vaginal Lavage Fluid
Applications: ELISA Development

Yano et al. (2012), The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway. PLoS ONE, 7(9):e46311.
PMID: 23050010
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Dessing MC et al. (2010), S100A8/A9 is not involved in host defense against murine urinary tract infection. PLoS ONE, 5(10):e13394.
PMID: 20976233

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Customer Reviews for Mouse S100A8 Antibody (1)

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Mouse S100A8 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Artery Smooth Muscle Cells,Pulmonary Artery Smooth Muscle Cells

Species: Mouse

Verified Customer | Posted 11/02/2021

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Mouse S100A8 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse S100A8 Antibody

Scientific Data Examples for Mouse S100A8 Antibody

Western Blot

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: S100A8

Citations for Mouse S100A8 Antibody (6)

Customer Reviews for Mouse S100A8 Antibody (1)

Customer Images

Mouse S100A8 Antibody

Product Documents for Mouse S100A8 Antibody

Product Datasheets

COA

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