Mouse/Rat Testisin/Prss21 Alexa Fluor™ Plus 555-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6820AFP555

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AF6820AFP555-100UG
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Polyclonal Goat IgG

View all Testisin/Prss21 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Testisin/Prss21

Specificity

Detects recombinant mouse Testisin/Prss21 in direct ELISAs and Western blots. Detects mouse and rat Testisin/Prss21 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Prss8 and rmPrss27 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Testisin/Prss21

Testisin is a serine protease encoded by the PRSS21 gene. Testisin is similar in structure to tryptases, and is also known as Tryptase‑4 (1). It is predicted to have substrate specificity similar to that of trypsin (2). Testisin is most highly expressed in testicular germ cells, but is also present in eosinophils (3). Testisin is known to be required for spermatogenesis, and deficiencies of the enzyme can result in infertility (4, 5). Testisin is anchored to the cell surface through a C-terminal glycosylphosphatidylinositol anchor (6). Recombinant mouse Testisin is expressed with a truncated C-terminus, resulting in its secretion. The recombinant protein was purified as the zymogen and can undergo autoactivation under the appropriate conditions. Over aa 28-298, mouse Testisin shares 92% aa identity with rat Testisin.

References

  1. Wong, G.W. et al. (2001) J. Biol. Chem. 276:20648.
  2. Hooper, J.D. et al. (2000) Biochim. Biophys. Acta 1492:63.
  3. Inoue, M. et al. (1998) Biochem. Biophys.  Res. Comm. 252:307.
  4. Netzel-Arnett, S. et al. (2009) Biol. Reprod. 81:921.
  5. Kawano, N. et al. (2010) Biol. Reprod. 83:359.
  6. Honda, A. et al. (2002) J. Biol. Chem. 277:16976.

Alternate Names

Prss21, TEST1

Entrez Gene IDs

10942 (Human); 57256 (Mouse)

Gene Symbol

PRSS21

UniProt

Q9JHJ7

Additional Testisin/Prss21 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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