Mouse/Rat DLL1 Antibody

Name: Mouse/Rat DLL1 Antibody
Brand: R&D Systems
SKU: AF3970

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF3970

Citations (5)

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Species Reactivity:	Mouse, Rat
Immunogen:	Mouse myeloma cell line NS0-derived recombinant rat DLL1 Gln18-Trp537 (predicted) Accession # P97677
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Sheep IgG
Specificity:	Detects rat and mouse DLL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human DLL‑1 is observed.

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Applications for Mouse/Rat DLL1 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Rat DLL1 Fc Chimera (Catalog # 3970-DL)
Flow Cytometry	1 µg/10⁶ cells	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse/Rat DLL1 Antibody

	Flow Cytometry Detection of DLL1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3970, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
	Immunohistochemistry DLL1 in Embryonic Mouse Stomach. DLL1 was detected in immersion fixed frozen sections of embryonic mouse stomach (E13.5) using 10 µg/mL Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3970) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature rat DLL1 consists of a 520 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, rat DLL1 shares 90% and 95% aa sequence identity with human and mouse DLL1, respectively. It shares 26%, 36%, and 53% aa sequence identity with rat DLL2, 3, and 4, respectively. The ADAM9, 12, or 17- mediated proteolysis of DLL1 releases a 60 kDa ECD fragment and regulates the Notch-dependent proliferation of hematopoietic and myogenic progenitor cells (2-4). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent
gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (5). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (6). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (4, 7-13). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (14). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (15-16).

Item	Value
References	Bettenhausen, B. et al. (1995) Development 121:2407. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. Karanu, F.N. et al. (2001) Blood 97:1960. Sun, D. et al. (2008) J. Cell Sci. 121:3815. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. Takahashi, Y. et al. (2003) Development 130:4259. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68. Kiernan, A.E. et al. (2005) Development 132:4353. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749. Hozumi, K. et al. (2004) Nat. Immunol. 5:638. Shimojo, H. et al. (2008) Neuron 58:52. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. USA 104:537. Limbourg, A. et al. (2007) Circ. Res. 100:363. Purow, B.W. et al. (2005) Cancer Res. 65:2353. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
Long Name	Delta-like 1
Entrez Gene IDs	28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names	DL1, DLL1, Delta, Delta 1, Delta1, Drosophila Delta homolog 1, H-Delta-1, delta (Drosophila)-like 1, delta-like 1 (Drosophila), delta-like protein 1

Citations for Mouse/Rat DLL1 Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat DLL1 Antibody include:

Species: Human
Sample Types: Organoid
Applications: IHC

G de Medeiro et al. (2022), Multiscale light-sheet organoid imaging framework Nature Communications, 13(1):4864.
PMID: 35982061
Species: Human
Sample Types: Lysosomes
Applications: ICC

YX Xie et al. (2022), Lysosomal exocytosis releases pathogenic alpha-synuclein species from neurons in synucleinopathy models Nature Communications, 13(1):4918.
PMID: 35995799
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

F Tetzlaff et al. (2018), MPDZ promotes DLL4-induced Notch signaling during angiogenesis Elife, 7(0).
PMID: 29620522
Species: Chicken
Sample Types: Whole Cells
Applications: IHC

Hatakeyama J et al. (2014), Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates. Development, 141(8):1671-82.
PMID: 24715457
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Kawaguchi A et al. (2008), Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis. Development, 135(18):3113-24.
PMID: 18725516

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Mouse/Rat DLL1 Antibody

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Applications for Mouse/Rat DLL1 Antibody

Scientific Data Examples for Mouse/Rat DLL1 Antibody

Flow Cytometry

Immunohistochemistry

Formulation, Preparation and Storage

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Background: DLL1

Citations for Mouse/Rat DLL1 Antibody (5)

Product Documents for Mouse/Rat DLL1 Antibody

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