Mouse/Rat DLL1 Alexa Fluor™ Plus 680-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3970AFP680

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AF3970AFP680-100UG
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Sheep IgG

View all DLL1 Antibodies »

Product Specifications

Specificity

Detects rat and mouse DLL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human DLL‑1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature rat DLL1 consists of a 520 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, rat DLL1 shares 90% and 95% aa sequence identity with human and mouse DLL1, respectively. It shares 26%, 36%, and 53% aa sequence identity with rat DLL2, 3, and 4, respectively. The ADAM9, 12, or 17- mediated proteolysis of DLL1 releases a 60 kDa ECD fragment and regulates the Notch-dependent proliferation of hematopoietic and myogenic progenitor cells (2-4). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent
gamma-secretase, enabling the cytoplasmic domain to migrate to the nucleus (5). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (6). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (4, 7-13). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (14). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (15-16).

References

  1. Bettenhausen, B. et al. (1995) Development 121:2407.
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
  3. Karanu, F.N. et al. (2001) Blood 97:1960.
  4. Sun, D. et al. (2008) J. Cell Sci. 121:3815.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
  7. Takahashi, Y. et al. (2003) Development 130:4259.
  8. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  9. Kiernan, A.E. et al. (2005) Development 132:4353.
  10. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  11. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  12. Shimojo, H. et al. (2008) Neuron 58:52.
  13. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. USA 104:537.
  14. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  15. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  16. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.

Long Name

Delta-like 1

Alternate Names

Delta 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Gene Symbol

DLL1

UniProt

P97677

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Certificate of Analysis

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Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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