RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific Monoclonal Antibody (Catalog # MAB17582, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Detection of Rae-1 Pan Specific by Flow Cytometry

NK cell mediated non-self or induced-self recognition does not impact the expansion of BCR-ABL1+ cells.(A) The bar graph shows the mean absolute number (±SD) of B6 Dd (H-2bDd, CD45.1)-derived myeloid cells expressing BCR-ABL1 (GFP) in B6 (H-2b, CD45.2) recipients at day 8 after transplantation (MHC-I different, non-self recognition). (B) The expression of Rae-1 NKG2D ligands was determined on B6 (left) or BALB.B (right)-derived myeloid cells (CD11b+) expressing BCR-ABL1 on day 8 after transplantation into B6 mice (gray fill). Open histograms depict background staining on BM-derived CD11b+ cells of normal B6 mice. (C) The bar graph shows the mean absolute number (±SD) of BABL.B (H-2b, CD45.2)-derived myeloid cells expressing GFP (either control or BCR-ABL1) in B6 (H-2b, CD45.1) recipients at day 8 after transplantation (MHC-I matched, unrelated, induced-self recognition). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22132120), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Rae-1 Pan Specific Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rae-1

Rae-1 alpha, beta, gamma, delta and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Long Name

Retinoic Acid Early Transcript 1

Alternate Names

Rae1

Entrez Gene IDs

8480 (Human); 19368 (Mouse)

Gene Symbol

RAE1

UniProt

Q9JI58

Additional Rae-1 Products

Product Documents for Mouse Rae-1 Pan Specific Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Rae-1 Pan Specific Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Rae-1 Pan Specific Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB17582

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Rae-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Rae-1 Pan Specific Antibody

Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.