Mouse Rae-1 alpha/ beta/ gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1758
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 199215
Product Specifications
Immunogen
BaF3 mouse pro-B cell line transfected with Rae-1 beta and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1 delta
Specificity
Detects mouse Rae‑1 alpha/ beta/ gamma. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody recognizes mouse Rae-1 alpha, beta and gamma. It does not recognize Rae-1 delta or epsilon.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse Rae-1 alpha/ beta/ gamma Antibody
Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 alpha/beta/gamma Monoclonal Antibody (Catalog # MAB1758, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse Rae-1 alpha/ beta/ gamma Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of Recombinant Mouse NKG2D Fc Chimera (Catalog # 139-NK) to immobilized Recombinant Mouse Rae-1 beta Fc Chimera (Catalog # 1198-RA) coated at 1 µg/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding. This antibody also blocks the binding of Recombinant Mouse NKG2D Fc Chimera to Recombinant Mouse Rae-1 alpha and Rae-1 gamma.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rae-1 alpha/beta/gamma
The Rae-1 family is a group of closely related GPI-linked proteins that function as ligands for NKG2D. Binding of Rae-1 proteins to NKG2D results in the activation of cytolytic activity and/or cytokine production by effector T cells and NK cells.
Long Name
Retinoic Acid Early Transcript 1
Gene Symbol
RAE1
Additional Rae-1 alpha/beta/gamma Products
Product Documents for Mouse Rae-1 alpha/ beta/ gamma Antibody
Product Specific Notices for Mouse Rae-1 alpha/ beta/ gamma Antibody
For research use only
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