Detects mouse PGLYRP2/PGRP-L in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) PGRP-S and rmPGRP-1 beta is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse PGLYRP2/PGRP-L Antibody

Detection of PGLYRP2/PGRP-L in Mouse Liver Cells by Flow Cytometry.

Mouse liver single-cell suspension was stained with Rat Anti-Mouse PGLYRP2/PGRP-L Monoclonal Antibody (Catalog # MAB4704, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Mouse PGLYRP2/PGRP-L Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse liver single-cell suspension fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Mouse PGLYRP2/PGRP-L

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGLYRP2/PGRP-L

PGRP-L (peptidoglycan recognition protein‑long; also called PGRP-2 or TagL) is a 530 amino acid (aa) member of the PGRP family that is expressed in the liver. All mouse PGRPs bind peptidoglycan and Gram-positive bacteria, and may function as innate immunity pattern recognition molecules. PGRP-L is a Zn²⁺-dependent N‑acetylmuramoyl-L-alanine amidase that hydrolyzes cell wall glycopeptides. It contains two membrane-spanning segments, with both termini thought to be extracellular, but is also reported in serum. A truncated 450 aa isoform has been reported. Overall aa identity between mouse and human PGRP-L is 68%.

Long Name

Peptidoglycan Recognition Protein Long/Peptidoglycan Recognition Protein 2

Alternate Names

PGLYRPL, PGRP-L, PGRP2, PGRPL, TAGL

Entrez Gene IDs

114770 (Human); 57757 (Mouse)

Gene Symbol

PGLYRP2

UniProt

Q8VCS0

Additional PGLYRP2/PGRP-L Products

Product Documents for Mouse PGLYRP2/PGRP-L Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Mouse PGLYRP2/PGRP-L Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse PGLYRP2/PGRP-L Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4704

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity