Mouse OX40 Ligand/TNFSF4 Antibody

Name: Mouse OX40 Ligand/TNFSF4 Antibody
Brand: R&D Systems
SKU: MAB1236

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1236

Citations (3)

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Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse OX40 Ligand/TNFSF4 Gln49-Leu198 Accession # P43488
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 182601
Specificity:	Detects mouse OX40 Ligand/TNFSF4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) APRIL, rhGITR Ligand, rhLIGHT, rhLT alpha 1/ beta 2, rhLT alpha 2/ beta 1, recombinant cotton rat TNF‑ alpha, recombinant mouse (rm) TNF‑ alpha, rmTRAIL, rhTRANCE, rmTRANCE, rmTWEAK, or rhVEGI is observed.

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Applications for Mouse OX40 Ligand/TNFSF4 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse OX40 Ligand/TNFSF4 (Catalog # 1236-OX) under non-reducing conditions only

Item	Value
Neutralization	Measured by its ability to neutralize OX40 Ligand/TNFSF4-induced IL‑2 secretion in mouse T cells. The Neutralization Dose (ND₅₀) is typically 0.01-0.05 µg/mL in the presence of 30 ng/mL Recombinant Mouse OX40 Ligand/TNFSF4, 10 µg/mL of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody, and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse OX40 Ligand/TNFSF4 Antibody

	Neutralization IL‑2 Secretion Induced by OX40 Ligand/TNFSF4 and Neutralization by Mouse OX40 Ligand/TNFSF4 Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050) and sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog # MAB484), Recombinant Mouse OX40 Ligand/TNFSF4 (Catalog # 1236-OX) stimulates IL-2 secretion in mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IL-2 Quantikine ELISA Kit (Catalog # M2000). Under these conditions, IL-2 secretion elicited by Recombinant Mouse OX40 Ligand/TNFSF4 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse OX40 Ligand/TNFSF4 Monoclonal Antibody (Catalog # MAB1236). The ND₅₀ is typically 0.01-0.05 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Murine OX40L cDNA encodes a 198 amino acid (aa) protein comprised of a 28 aa N-terminal cytoplasmic domain, a 20 aa transmembrane segment, and a 150 aa C-terminal extracellular domain (1). Human and mouse OX40L share 46% sequence identity at the amino acid level (1). OX40L is expressed on activated antigen presenting cells such as B cells, macrophages, dendritic cells, and on endothelial cells at the site of inflammation. The receptor for OX40L is OX40 (CD134) which is expressed predominantly on activated CD4⁺T cells. Expression of OX40 is transient following engagement of T cell receptors (2). Ligation of OX40L by OX40 stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion (3, 4). The expression of OX40L on B cells is upregulated by CD40 ligation (3). Engagement of the OX40‑OX40L system has co-stimulatory effects on T cells by stimulating the production of cytokines by T helper cells and increasing the survival of memory T cells (2, 5). Blocking of the OX40‑OX40L interaction in vitro inhibits co-stimulation resulting in decreased T cell proliferation and adhesion of T cells to endothelial cells. Inhibition of the OX40‑OX40L interaction in disease models has beneficial effects in acute graft-versus-host disease, inflammatory bowel disease and decreases the development of collagen-induced arthritis and experimental leishmaniasis (6).

Item	Value
References	Baum, P.R. et al. (1994) EMBO J. 13:3992. Gramaglia, I. et al. (1999) J. Immunol. 161:6510. Stuber, E. et al. (1995) Immunity 2:507. Malstrom, V. et al. (2001) J. Immunol. 166:6972. Maxwell, J.R. et al. (2000) J. Immunol. 164:107. Weinberg, A.D. (2002) Trends Immunol. 23:102.
Entrez Gene IDs	7292 (Human); 22164 (Mouse); 89814 (Rat)
Alternate Names	CD134 ligand, CD134L, CD252, CD252 antigen, Glycoprotein Gp34, OX-40L, OX40 Ligand, OX40 antigen ligand, OX40L, OX4OL, TAX transcriptionally-activated glycoprotein 1, TNFSF4, TXGP1, gp34, tax-transcriptionally activated glycoprotein 1 (34kD), tax-transcriptionally activated glycoprotein 1, 34kD, tumor necrosis factor (ligand) superfamily, member 4, tumor necrosis factor ligand superfamily member 4

Citations for Mouse OX40 Ligand/TNFSF4 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse OX40 Ligand/TNFSF4 Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

L Sun et al. (2018), TSLP-activated dendritic cells induce T helper type 2 inflammation in Aspergillus fumigatus keratitis Exp. Eye Res., 0(0).
PMID: 29550278
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Yokota-Nakatsuma A et al. (2014), Retinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells. Mucosal Immunol, 7(4):786-801.
PMID: 24220301
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Peters M et al. (2010), T-cell polarization depends on concentration of the danger signal used to activate dendritic cells. Immunol. Cell Biol., 88(5):537-44.
PMID: 20125117

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Mouse OX40 Ligand/TNFSF4 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse OX40 Ligand/TNFSF4 Antibody

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Neutralization

Formulation, Preparation and Storage

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Background: OX40 Ligand/TNFSF4

Citations for Mouse OX40 Ligand/TNFSF4 Antibody (3)

Product Documents for Mouse OX40 Ligand/TNFSF4 Antibody

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