M1 mouse myeloid leukemia cell line was stained with Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

MICL/CLEC12A in Mouse Splenocytes.

MICL/CLEC12A was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MICL/CLEC12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2950) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse MICL/CLEC12A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: M1 mouse myeloid leukemia cell line

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse MICL/CLEC12A

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICL/CLEC12A

MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.

Long Name

Myeloid Inhibitory C-type Lectin-like Receptor

Alternate Names

CD371, CLEC12A, CLL-1, DCAL-2

Entrez Gene IDs

160364 (Human); 232413 (Mouse)

Gene Symbol

CLEC12A

UniProt

NP_808354

Additional MICL/CLEC12A Products

Product Documents for Mouse MICL/CLEC12A Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse MICL/CLEC12A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse MICL/CLEC12A Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2950

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all MICL/CLEC12A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse MICL/CLEC12A Antibody

Detection of MICL/CLEC12A in M1 Mouse Cell Line by Flow Cytometry.