Mouse IL-1ra/IL-1F3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-480-NA
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse IL‑1ra/IL-1F3
Specificity
Detects mouse IL-1ra/IL-1F3 in ELISAs and Western blots. In sandwich ELISAs, approximately 45% cross-reactivity with recombinant rat IL‑1ra/IL‑1F3 and less than 1% cross-reactivity with recombinant human IL‑1ra/IL‑1F3, recombinant porcine IL‑1ra/IL‑1F3, and recombinant equine IL‑1ra/IL‑1F3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IL-1ra/IL-1F3 Antibody
IL-1ra/IL-1F3 Inhibition of IL-1 alpha/IL-1F1-dependent Cell Proliferation and Neutralization by Mouse IL-1ra/IL-1F3 Antibody.
Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM) inhibits Recombinant Human IL-1a/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1a/IL-1F1 (50 pg/mL) activity elicited by Recombinant Mouse IL-1ra/IL-1F3 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA). The ND50 is typically 1-5 µg/mL.
Mouse IL-1ra / IL-1F3 ELISA Standard Curve
Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM) was serially diluted and captured by Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Mouse IL-1ra / IL-1F3 ELISA Standard Curve
Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM) was serially diluted and captured by Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-480-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse IL-1ra/IL-1F3 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)
Sample: Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)
Neutralization
Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha/IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line [Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272]. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 150 ng/mL Recombinant Mouse IL‑1ra/IL‑1F3, 50 pg/mL Recombinant Human IL‑1 alpha/IL‑1F1.
Mouse IL-1ra/IL-1F3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1ra/IL-1F3
IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.
Long Name
Interleukin 1 Receptor Antagonist
Alternate Names
DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4
Gene Symbol
IL1RN
Additional IL-1ra/IL-1F3 Products
Product Documents for Mouse IL-1ra/IL-1F3 Antibody
Product Specific Notices for Mouse IL-1ra/IL-1F3 Antibody
For research use only
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