Recombinant Human IL-1 alpha/IL-1F1 Protein Best Seller
Catalog # 200-LA | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Ser113-Ala271
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 1‑6 pg/mL.
Scientific Data Images for Recombinant Human IL-1 alpha/IL-1F1 Protein
Recombinant Human IL-1 alpha/IL-1F1 Protein Bioactivity
Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 1-6 pg/mL.Recombinant Human IL-1 alpha/IL-1F1 Protein SDS-PAGE
1 μg/lane of Recombinant Human IL-1 alpha/IL-1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 200-LA
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 200-LA/CF
Formulation | Supplied as a 0.2 μm filtered solution in PBS. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IL-1 alpha/IL-1F1
Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).
IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.
Product Documents for Recombinant Human IL-1 alpha/IL-1F1 Protein
Product Specific Notices for Recombinant Human IL-1 alpha/IL-1F1 Protein
For research use only
Citations for Recombinant Human IL-1 alpha/IL-1F1 Protein (59)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-1 alpha/IL-1F1 Protein include:
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Species: Human
Sample Types: Whole Cells
Applications: BioassayKarema-Jokinen et al. (2023-05-01), Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients The Journal of biological chemistry
PMID: 37137441 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayP Szpakowski et al. (2022-07-22), Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli Biomedicines, 2022-010(8).
PMID: 35892669 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayMC Ebeling et al. (2022-06-30), Inflammasome Activation in Retinal Pigment Epithelium from Human Donors with Age-Related Macular Degeneration Cells, 2022-011(13).
PMID: 35805159 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayE Filidou et al. (2022-04-20), Anti-Inflammatory and Anti-Fibrotic Effect of Immortalized Mesenchymal-Stem-Cell-Derived Conditioned Medium on Human Lung Myofibroblasts and Epithelial Cells International Journal of Molecular Sciences, 2022-023(9).
PMID: 35562961 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureY Zhu et al. (2022-03-13), Orally-active, clinically-translatable senolytics restore alpha-Klotho in mice and humans EBioMedicine, 2022-00(0):103912.
PMID: 35292270 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM McAleavy et al. (2021-11-01), The Activin/FLRG pathway associates with poor COVID-19 outcomes in hospitalized patients Molecular and Cellular Biology, 2021-10(0):MCB0046721.
PMID: 34723652 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayH Sheldon et al. (2021-10-19), ELTD1 Activation Induces an Endothelial-EMT Transition to a Myofibroblast Phenotype International Journal of Molecular Sciences, 2021-122(20).
PMID: 34681953 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayR Biavasco et al. (2021-07-27), Oncogene-induced senescence in hematopoietic progenitors features myeloid restricted hematopoiesis, chronic inflammation and histiocytosis Nature Communications, 2021-012(1):4559.
PMID: 34315896 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayN Bhattarai et al. (2021-06-06), Hydroquinone Induces NLRP3-Independent IL-18 Release from ARPE-19 Cells Cells, 2021-010(6).
PMID: 34204067 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Ranta-Aho et al. (2021-05-05), TAS-116, a Well-Tolerated Hsp90 Inhibitor, Prevents the Activation of the NLRP3 Inflammasome in Human Retinal Pigment Epithelial Cells International Journal of Molecular Sciences, 2021-022(9).
PMID: 34062977 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Hayn et al. (2021-04-27), Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities Cell Reports, 2021-035(7):109126.
PMID: 33974846 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayK Bernau et al. (2021-03-02), Interleukin-1&alpha Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation Cells, 2021-010(3).
PMID: 33801398 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureX Li et al. (2020-11-13), 18F-FHBG PET-CT Reporter Gene Imaging of Adoptive CIK Cell Transfer Immunotherapy for Breast Cancer in a Mouse Model Onco Targets Ther, 2020-113(0):11659-11668.
PMID: 33223839 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayD Liu et al. (2020-10-22), Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma Biomedicines, 2020-18(11).
PMID: 33105540 -
Species: Mouse
Sample Types: Reference Standard
J Sun et al. (2020-09-23), The Pseudomonas aeruginosa protease LasB directly activates IL-1&beta EBioMedicine, 2020-060(0):102984.
PMID: 32979835 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayCJ Cunningham et al. (2020-01-21), Systemic conditioned medium treatment from interleukin-1 primed mesenchymal stem cells promotes recovery after stroke Stem Cell Res Ther, 2020-011(1):32.
PMID: 31964413 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayNE Powers et al. (2019-12-09), PASylation of IL-1 Receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis J. Biol. Chem., 2019-10(0).
PMID: 31819009 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayN Piippo et al. (2018-08-23), Oxidative Stress is the Principal Contributor to Inflammasome Activation in Retinal Pigment Epithelium Cells with Defunct Proteasomes and Autophagy Cell. Physiol. Biochem., 2018-049(1):359-367.
PMID: 30138927 -
Species: Bovine
Sample Types: Whole Tissue
Applications: BioassayKC Ravindra et al. (2018-05-23), Chemoproteomics of matrix metalloproteases in a model of cartilage degeneration suggests functional biomarkers associated with posttraumatic osteoarthritis J. Biol. Chem., 2018-00(0).
PMID: 29794029 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayE Couderc et al. (2017-07-14), Interleukin-17A-induced production of acute serum amyloid A by keratinocytes contributes to psoriasis pathogenesis PLoS ONE, 2017-012(7):e0181486.
PMID: 28708859 -
Species: Human
Sample Types: Plasma
Applications: ControlJH von Steman et al. (2017-06-30), Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study PLoS ONE, 2017-012(6):e0179981.
PMID: 28665954 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayB Tian et al. (2017-05-24), Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells Sci Rep, 2017-07(1):2329.
PMID: 28539592 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayE Redondo-Ca et al. (2017-04-17), Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro Stem Cell Res Ther, 2017-08(1):79.
PMID: 28412968 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayKM Perrott et al. (2017-04-04), Apigenin suppresses the senescence-associated secretory phenotype and paracrine effects on breast cancer cells Geroscience, 2017-039(2):161-173.
PMID: 28378188 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySojoong Choi (2016-12-28), Inflammatory hypoxia induces syndecan-2 expression through IL-1?-mediated FOXO3a activation in colonic epithelia FASEB J, 2016-10(0).
PMID: 28031321 -
Species: Human
Sample Types: Whole Cells
Applications: Bioassay(2016-09-20), Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients PLoS ONE, 2016-011(9):e0162908.
PMID: 27649540 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayMD Ballantyne et al. (2016-04-06), Smooth Muscle Enriched Long Non-Coding RNA (SMILR) Regulates Cell Proliferation Circulation, 2016-00(0).
PMID: 27052414 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Robert et al. (2016-04-05), Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1? or TNF-? Release from Human Hepatic Stellate Cells PLoS ONE, 2016-011(4):e0153118.
PMID: 27046197 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayDankbar B et al. (2015-08-03), Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice. Nat Med, 2015-021(9):1085-90.
PMID: 26236992 -
Species: Rat
Sample Types: In Vivo
Applications: In VivoFernandez-Bustamante A et al. (2015-07-06), Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats. PLoS ONE, 2015-010(7):e0130764.
PMID: 26147379 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayLaberge R et al. (2015-07-06), MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol, 2015-017(8):1049-61.
PMID: 26147250 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayRabeony H et al. (2014-07-10), Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M. PLoS ONE, 2014-09(7):e101937.
PMID: 25010647 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGschwandtner M et al. (2014-04-02), Fetal human keratinocytes produce large amounts of antimicrobial peptides: involvement of histone-methylation processes. J Invest Dermatol, 2014-0134(8):2192-201.
PMID: 24694903 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGieseke F et al. (2013-07-23), Proinflammatory stimuli induce galectin-9 in human mesenchymal stromal cells to suppress T-cell proliferation. Eur J Immunol, 2013-043(10):2741-9.
PMID: 23817958 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayWilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448 -
Species: Human
Sample Types: Whole Cells
Applications: Bioassay(2012-07-16), Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J. Exp. Med., 2012-0209(8):1505-17.
PMID: 22802353 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJung DW et al. (2010-07-15), Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int. J. Cancer, 2010-0127(2):332-44.
PMID: 19937793 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGudjonsson JE et al. (2010-04-08), Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 2010-0130(7):1849-59.
PMID: 20376066 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGuilloteau K et al. (2010-03-24), Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis. J. Immunol., 2010-0184(0):5263-70.
PMID: 20335534 -
Sample Types: N/A
Applications: ELISA (Standard)Gonzalez RM et al. (2008-04-19), Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-07(6):2406-14.
PMID: 18422355 -
Sample Types: N/A
Applications: ELISA (Standard)Biancotto A et al. (2008-03-01), Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-024(3):453-62.
PMID: 18327985 -
Sample Types: N/A
Applications: ELISA (Standard)Pfleger C et al. (2007-10-22), Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-1329(1):214-8.
PMID: 17964594 -
Sample Types: N/A
Applications: ELISA (Standard)Todd J et al. (2007-09-21), Ultrasensitive flow-based immunoassays using single-molecule counting. Clin. Chem., 2007-053(11):1990-5.
PMID: 17890441 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayKelley MJ et al. (2007-06-01), Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork. Invest. Ophthalmol. Vis. Sci., 2007-048(6):2634-43.
PMID: 17525194 -
Sample Types: N/A
Applications: Western BlotWilson HL et al. (2007-03-12), P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells. Br. J. Pharmacol., 2007-0151(1):115-27.
PMID: 17351655 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayBrechter AB et al. (2007-03-01), Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression. Arthritis Rheum., 2007-056(3):910-23.
PMID: 17328065 -
Sample Types: N/A
Applications: ELISA (Standard)Biancotto A et al. (2007-02-08), Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-0109(10):4272-9.
PMID: 17289812 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGleissner CA et al. (2007-01-01), IL-10 inhibits endothelium-dependent T cell costimulation by up-regulation of ILT3/4 in human vascular endothelial cells. Eur. J. Immunol., 2007-037(1):177-92.
PMID: 17163451 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJohnson LA et al. (2006-11-20), An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J. Exp. Med., 2006-1203(12):2763-77.
PMID: 17116732 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayAbe Y et al. (2006-10-04), L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion. Atherosclerosis, 2006-1192(1):56-66.
PMID: 17022986 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayLi Y et al. (2006-05-11), Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration. J. Invest. Dermatol., 2006-0126(9):2096-105.
PMID: 16691197 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayPalmer G et al. (2005-07-21), Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1). Cytokine, 2005-031(2):153-60.
PMID: 15923123 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayDulos J et al. (2005-03-01), CYP7B expression and activity in fibroblast-like synoviocytes from patients with rheumatoid arthritis: regulation by proinflammatory cytokines. Arthritis Rheum., 2005-052(3):770-8.
PMID: 15751070 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayKao CY et al. (2004-09-01), IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J. Immunol., 2004-0173(5):3482-91.
PMID: 15322213 -
Species: Bovine
Sample Types: Whole Cells
Applications: BioassayMorita K et al. (2004-08-04), Interleukin-1 inhibits voltage-dependent P/Q-type Ca2+ channel associated with the inhibition of the rise of intracellular free Ca2+ concentration and catecholamine release in adrenal chromaffin cells. Biochim. Biophys. Acta, 2004-01673(3):160-9.
PMID: 15279887 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayKawano Y et al. (2004-08-01), The effect of inflammatory cytokines on secretion of macrophage colony-stimulating factor and monocyte chemoattractant protein-1 in human granulosa cells. Am. J. Reprod. Immunol., 2004-052(2):124-8.
PMID: 15274652 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySmith DE et al. (2003-01-01), The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action. Immunity, 2003-018(1):87-96.
PMID: 12530978 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySzabowski A et al. (2000-11-22), c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cell, 2000-1103(5):745-55.
PMID: 11114331 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayLerner UH et al. (1998-06-01), Gingival crevicular fluid from patients with periodontitis contains bone resorbing activity. Eur. J. Oral Sci., 1998-0106(3):778-87.
PMID: 9672100
There are no citations that match your criteria.
Customer Reviews for Recombinant Human IL-1 alpha/IL-1F1 Protein (4)
Customer Images
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 09/16/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 04/13/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 12/22/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 05/14/2019
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