Mouse IL-17RB Biotinylated Antibody

Name: Mouse IL-17RB Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1040

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF1040

Citations (5)

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Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RB Arg18-Gly286 Accession # Q9JIP3
Applications:	ELISA Detection, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse IL‑17 RB in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human IL‑17 RB, recombinant mouse (rm) IL-17 and rmIL-17 R is observed.

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Applications for Mouse IL-17RB Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑17 RB Fc Chimera (Catalog # 1040-BR)

Mouse IL-17 RB Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse IL-17RB Antibody (Catalog # MAB10401)

Standard: Recombinant Mouse IL-17RB Fc Chimera Protein, CF (Catalog # 1040-BR)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine production and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1, 2). Interleukin-17B Receptor (IL-17RB), also known as IL-17 Rh1, IL-17E R and EVI27, represents the second receptor of the IL-17 family cytokines to be recognized (2‑4). Mouse IL17B R cDNA encodes a 499 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 269 aa extracellular domain, a 21 aa transmembrane domain and a 192 aa cytoplasmic tail. As a result of alternative splicing, a secreted variant of IL-17B R also exists (4). Human and mouse IL‑17B R share 76% aa sequence identity. The IL-17B R protein sequence is only 19.2% and 18.2% identical to the human and mouse IL-17 R sequence, respectively. However, the receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. At least three additional type I transmembrane receptors with homology to IL‑17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have been reported (2, 6). By Northern blot analysis, mouse IL-17B R is highly expressed in liver and testes and is expressed at lower levels in kidney and lung. It is also expressed in some hematopoietic cell lines, including selected T cell, B cell, and myeloid cell lines (2‑4). The expression of IL-17B R is significantly up‑regulated under inflammatory conditions (7). IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17 or IL-17F. Activation of IL-17B R by its ligands results in the activation of nuclear factor kappa-B (2‑4).

Item	Value
References	Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167. Lee, J. et al. (2001) J. Biol. Chem. 276:1660. Tian, E. et al. (2000) Oncogene 19:2098. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Long Name	Interleukin 17B Receptor
Entrez Gene IDs	55540 (Human); 50905 (Mouse)
Alternate Names	CRL4IL-17 receptor homolog 1, Cytokine receptor-like 4, EVI27IL-17B receptor, Evi27, IL-17 RB, IL-17 RH1, IL-17 receptor B, IL-17ER, IL-17RB, IL-17Rh1, IL17BRinterleukin 17B receptor, IL17RB, IL17RH1MGC5245, IL17Rh1, Interleukin-17B receptor, cytokine receptor CRL4, interleukin 17 receptor B, interleukin 17 receptor homolog 1, interleukin-17 receptor B

Citations for Mouse IL-17RB Biotinylated Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-17RB Biotinylated Antibody include:

Species: Mouse
Sample Types: Protein
Applications: Western Blot

S Li et al. (2022), IL-25 improves diabetic wound healing through stimulating M2 macrophage polarization and fibroblast activation International immunopharmacology, 106(0):108605.
PMID: 35149293
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

T Yoshizaki et al. (2019), IL-25 exacerbates autoimmune aortitis in IL-1 receptor antagonist-deficient mice Sci Rep, 9(1):17067.
PMID: 31745167
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

H Suto et al. (2018), IL-25 enhances Th17 cell-mediated contact dermatitis by promoting IL-1? production by dermal dendritic cells J. Allergy Clin. Immunol., 0(0).
PMID: 29522843
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Sawa S et al. (2011), RORgammat+ innate lymphoid cells regulate intestinal homeostasis by integrating negative signals from the symbiotic microbiota. Nat. Immunol., 12(4):320-6.
PMID: 21336274
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Bartemes KR et al. (2011), IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs. J. Immunol., 188(3):1503-13.
PMID: 22198948

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Showing 1 - 5 of 5 citations Showing All

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Mouse IL-17RB Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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