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Recombinant Mouse IL-17RB Fc Chimera Protein, CF

Catalog # 1040-BR | R&D Systems, Inc. a Bio-Techne Brand
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1040-BR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-17 RB protein
Mouse IL-17 RB
(Arg18-Gly286)
Accession # Q9JIP3
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg18

Predicted Molecular Mass

56 kDa (monomer)

SDS-PAGE

70-75 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse IL-17E/IL-25 (Catalog # 1399-IL) is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Mouse IL-17 RB Fc Chimera that produces 50% of the optimal binding response is approximately 2-12 ng/mL.

Formulation, Preparation and Storage

1040-BR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine production and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1, 2).

Interleukin-17B Receptor (IL-17B R), also known as IL-17 Rh1, IL-17E R and EVI27, represents the second receptor of the IL-17 family cytokines to be recognized (2‑4). Mouse IL17B R cDNA encodes a 499 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 269 aa extracellular domain, a 21 aa transmembrane domain and a 192 aa cytoplasmic tail. As a result of alternative splicing, a secreted variant of IL-17B R also exists (4). Human and mouse IL‑17B R share 76% aa sequence identity. The IL-17B R protein sequence is only 19.2% and 18.2% identical to the human and mouse IL-17 R sequence, respectively. However, the receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. At least three additional type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have been reported (2, 6). By Northern blot analysis, mouse IL-17B R is highly expressed in liver and testes and is expressed at lower levels in kidney and lung. It is also expressed in some hematopoietic cell lines, including selected T cell, B cell, and myeloid cell lines (2 - 4). The expression of IL-17B R is significantly up-regulated under inflammatory conditions (7). IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17 or IL-17F. Activation of IL-17B R by its ligands results in the activation of nuclear factor kappa-B (2-4).

References

  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
  4. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  5. Tian, E. et al. (2000) Oncogene 19:2098.
  6. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  7. Hurst, S.D. et al. (2002) J. Immunol. 169:443.

Long Name

Interleukin 17B Receptor

Alternate Names

Evi27, IL-17 RB, IL-17 RH1, IL-17ER, IL17RB

Entrez Gene IDs

55540 (Human); 50905 (Mouse)

Gene Symbol

IL17RB

UniProt

Product Documents for Recombinant Mouse IL-17RB Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IL-17RB Fc Chimera Protein, CF

For research use only

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