Mouse IL-12 R beta 2 Antibody

Name: Mouse IL-12 R beta 2 Antibody
Brand: R&D Systems
SKU: MAB8650

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB8650

Citations (2)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse IL-12 R beta 2 Met1-Asn637 Accession # P97378
Applications:	Block/Neutralize
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2B Clone # 918102
Specificity:	Detects mouse IL-12 R beta 2 in direct ELISAs.

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Applications for Mouse IL-12 R beta 2 Antibody

	Recommended Concentration	Sample
Blockade of Receptor-ligand Interaction	In a functional ELISA, 30-180 ng/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Mouse IL‑12 (Catalog # 419-ML) to immobilized Recombinant Mouse IL-12 R beta 2 Fc Chimera (Catalog # 7406-MR) coated at 500 ng/mL (100 μL/well). At 1 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-12 R beta 2

The high‑affinity IL‑12 receptor complex includes the 100 kDa IL‑12 receptor beta 1 (IL‑12 R beta 1) and the 130 kDa IL‑12 Receptor beta 2 (IL‑12 R beta 2) subunits, both of which are type I transmembrane proteins that belong to the cytokine receptor superfamily (1, 2). The complex's ligand, IL‑12, is a disulfide‑linked heterodimer composed of 35 kDa (IL‑12 alpha p35) and 40 kDa (IL‑12 beta p40) subunits. IL‑12 R beta 2 binds IL‑12 alpha and signals through Jak2, while IL‑12 R beta 1 binds IL‑12 beta and signals through Tyk2 (3).
IL‑12 R beta 1 is also a subunit of the IL‑23 receptor complex (3). The 874 amino acid (aa) mouse IL‑12 R beta 2 precursor includes a 23 aa signal peptide, a 614 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 216 aa cytoplasmic region. The ECD possesses one C2‑type Ig‑like domain, five fibronectin type III (Fn III) repeats, 14 potential N‑glycosylation sites, and a WSXWS motif, while the cytoplasmic region contains a Box 1 motif and three tyrosine phosphorylation sites that presumably mediate intracellular signaling (3). The mouse IL‑12 R beta 2 ECD shares 91% aa sequence identity with rat IL‑12 R beta 2, and 68% with human, porcine and bovine IL‑12 R beta 2. Human and mouse IL‑12 R beta 2 do not bind cross‑species IL‑12 (2). A 734 aa mouse isoform that lacks aa 363-503 within the Fn III domains is reported (4). Unlike IL‑12 R beta 1, which is constitutively expressed on T cells, NK cells and B cells, IL‑12 R beta 2 expression is more restricted (2). On naïve T cells, IL‑12 R beta 2 is expressed following STAT1 activation by IFN‑ gamma, IL‑27 and/or T cell receptor ligation. This up‑regulation allows IL‑12 to promote Th1, but not Th2, differentiation (5-7). Among B cells, surface expression is limited to naïve germinal center and memory B cells, and myeloma cells (2). Deletion of IL‑12 R beta 2 causes systemic overexpression of IL‑6, accelerated maturation of thymocytes, deficient regulatory T cell maturation and function, and reduced splenic T cell apoptosis (2, 8-10). These mice are susceptible to autoimmune diseases such as experimental autoimmune encephalitis and spontaneous B cell malignancies
(2, 8-10). In humans, polymorphism of the IL‑12 R beta 2 gene is associated with systemic sclerosis (11).

Item	Value
References	Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:14002. Pistoia, V. et al. (2009) J. Clin. Oncol. 27:4809. Zou, J. et al. (1997) J. Biol. Chem. 272:6073. GenBank protein Accession # P97378. Rogge, L. et al. (1997) J. Exp. Med. 185:825. Becskei, A. and M.J. Grusby (2007) FEBS Lett. 581:5199. Szabo, S.J. et al. (1997) J. Exp. Med. 185:817. Zhao, Z. et al. (2008) J. Immunol. 181:3870. Gran, B. et al. (2010) Exp. Mol. Pathol. 89:126. Airoldi, I. et al. (2005) Blood 106:3846. Bossini-Castillo, L. et al. (2011) Hum. Mol. Genet. Nov. 29 [Epub ahead of print].
Long Name	Interleukin 12 Receptor beta 2
Entrez Gene IDs	3595 (Human); 16162 (Mouse)
Alternate Names	IL-12 R beta 2, IL-12 receptor beta 2, IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, IL-12Rb2, IL12R beta 2, IL12RB2, interleukin 12 receptor, beta 2, interleukin-12 receptor beta-2 chain, interleukin-12 receptor subunit beta-2

Citations for Mouse IL-12 R beta 2 Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-12 R beta 2 Antibody include:

Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

J Nagai et al. (2019), P2Y6 signaling in alveolar macrophages prevents leukotriene-dependent type 2 allergic lung inflammation J. Clin. Invest., 0(0).
PMID: 31638598
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Tahseen H Nasti (2016), IL-23 Inhibits Melanoma Development by Augmenting DNA Repair and Modulating T Cell Subpopulations J. Immunol, 0(0).
PMID: 28003381

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