Mouse IL-1 RI Alexa Fluor™ Plus 680-conjugated Antibody

The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily (1 - 3). IL-1 RI is an ~80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein (1, 2). This complex recruits the adaptor protein MyD88, to initiate signaling in the NF kappaB pathway (4, 5). Mouse IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Mouse IL-1 RI shares 64%, 83%, 60%, 61% and 55% aa identity with human, rat, canine, equine and bovine IL-1 RI, respectively. The role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta, expression of IL-1 RI and its accessory and adaptor proteins, and negative regulators such as the IL‑receptor family member, IL-1RII/IL-1R2 (1 - 5). IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever (1, 2, 5, 6).