Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and rmICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, rrICAM-1, rmMAdCAM-1, or rmVCAM-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse ICAM-1/CD54 Antibody

Mouse ICAM-1 / CD54 ELISA Standard Curve

Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog # 796-IC) was serially diluted and captured by Rat Anti-Mouse ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB796) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse ICAM-1/CD54 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog # 796-IC)

Mouse ICAM-1/CD54 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse ICAM-1/CD54 Biotinylated Antibody (Catalog # BAF796)
Standard: Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF (Catalog # 796-IC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Mouse and human ICAM-1 share approximately 54% amino acid sequence identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

References

Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
Siu, G. et al. (1989) J. Immunol. 143:3813.
Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

3383 (Human); 15894 (Mouse); 25464 (Rat)

Gene Symbol

ICAM1

UniProt

P13597

Additional ICAM-1/CD54 Products

Product Documents for Mouse ICAM-1/CD54 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse ICAM-1/CD54 Antibody

For research use only

Citations
Reviews

Mouse ICAM-1/CD54 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB796

Key Product Details

Species Reactivity

Validated:

Cited:

Applications