Mouse Granzyme B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1865
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Xenograft
Applications
Validated:
Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Immunocytochemistry, Immunoprecipitation
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, ELISA Development (Capture), Functional Assay
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Granzyme B
Lys17-Ser247
Accession # P04187
Lys17-Ser247
Accession # P04187
Specificity
Detects mouse Granzyme B in ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Granzyme B Antibody
Granzyme B in Mouse Splenocytes.
Granzyme B was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Granzyme B in Mouse Spleen Tissue.
Granzyme B was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 8 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Mouse Granzyme B ELISA Standard Curve
Recombinant Mouse Granzyme B (Catalog # 1865-SE) was serially diluted and captured by Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse Granzyme B Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA
Sample: Immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen tissue
Sample: Immersion fixed paraffin-embedded sections of mouse spleen tissue
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Granzyme B (Catalog # 1865-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Granzyme B (Catalog # 1865-SE), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Granzyme B (Catalog # 1865-SE)
Sample: Recombinant Mouse Granzyme B (Catalog # 1865-SE)
Neutralization
Measured by its ability to neutralize Recombinant Mouse Granzyme B (0.5 µg/mL, Catalog # 1865-SE) cleavage of the fluorogenic peptide substrate Boc-AAD-SBzl (100 µM). The Neutralization Dose (ND50) is typically 3.5 µg/mL.
Mouse Granzyme B Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF1865 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Granzyme B
Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.
References
- Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
- Lobe, C.G. et al. (1986) Science 232:858.
- Brunet, J.F. et al. (1986) Nature 322:268.
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
UniProt
Additional Granzyme B Products
Product Documents for Mouse Granzyme B Antibody
Product Specific Notices for Mouse Granzyme B Antibody
For research use only
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