Mouse Galectin-1 Antibody

Name: Mouse Galectin-1 Antibody
Brand: R&D Systems
SKU: AF1245
Rating: 5 (5 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1245

Citations (16)

(5)

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Species Reactivity:	Mouse
Immunogen:	E. coli-derived recombinant mouse Galectin-1 Ala2-Glu135 Accession # P16045
Applications:	CyTOF-ready, ELISA Capture, Flow Cytometry, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse Galectin-1 in ELISAs and Western blots. In sandwich ELISAs, less than 5% cross-reactivity with recombinant human (rh) Galectin‑1 and less than 0.5% cross-reactivity with recombinant mouse (rm) Galectin-3, rhGalectin-4, rmGalectin-7, and rhGalectin‑8 is observed.

View Full Technical Details for Mouse Galectin-1 Antibody

Applications for Mouse Galectin-1 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	THP‑1 human acute monocytic leukemia cell line, L6 rat myoblast cell line, C2C12 mouse myoblast cell line, Neuro‑2A mouse neuroblastoma cell line and Mouse Thymus
Simple Western	5 µg/mL	L6 rat myoblast cell line, C2C12 mouse myoblast cell line, THP‑1 human acute monocytic leukemia cell line and Neuro‑2A mouse neuroblastoma cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/10⁶ cells	See below

Mouse Galectin-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse Galectin‑1 Biotinylated Antibody (Catalog # BAF1245)

Standard: Recombinant Mouse Galectin-1 Protein (Catalog # 1245-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse Galectin-1 Antibody

	Immunohistochemistry Galectin‑1 in Mouse Thymus. Galectin‑1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Intracellular Staining by Flow Cytometry Detection of Galectin‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
	Simple Western Detection of Mouse Galectin‑1 by Simple Western^TM. Simple Western lane view shows lysates of L6 rat myoblast cell line, C2C12 mouse myoblast cell line, THP‑1 human acute monocytic leukemia cell line and Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 18 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
	Western Blot Detection of Mouse Galectin‑1 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, L6 rat myoblast cell line, C2C12 mouse myoblast cell line, Neuro‑2A mouse neuroblastoma cell line and Mouse Thymus. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Galectin-1

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Mouse Galectin-1, also known as beta-galactoside-binding lectin L-14-I, lactose-binding lectin 1, S-Lac lectin 1, galaptin and 14 kDa lectin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, lymph nodes, spleen, thymus, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth, proliferation and differentiation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Mouse and human Galectin-1 share about 88% amino acid sequence similarity (1-5).

Item	Value
References	Rabinovich, A. et al. (2002) Trends in Immunol. 23:313. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741. Hughes, R.C. (2001) Biochimie 83:667. R&D Systems’ Cytokine Bulletin, Summer (2002). Goldring, K. et al. (2002) J. Cell Science 115:355.
Entrez Gene IDs	3956 (Human); 16852 (Mouse)
Alternate Names	14 kDa laminin-binding protein, 14 kDa lectin, BHL, Beta-galactoside-binding lectin L-14-I, DKFZp686E23103, GAL1, GBP, Galaptin, Galectin-1, Galectin1, HBL, HLBP14, HPL, L-14, LGALS1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, gal-1, galectin 1, lectin, galactoside-binding, soluble, 1

Citations for Mouse Galectin-1 Antibody (16)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Galectin-1 Antibody include:

Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

V Baloche et al. (2021), Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model Scientific Reports, 11(1):5227.
PMID: 33664349
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Capture

LG Morosi et al. (2021), Control of intestinal inflammation by glycosylation-dependent lectin-driven immunoregulatory circuits Science Advances, 7(25).
PMID: 34144987
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

HL Law et al. (2020), A Pro-resolving Role for Galectin-1 in Acute Inflammation Front Pharmacol, 11(0):274.
PMID: 32265698
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

JY Ge et al. (2020), Hepatic stellate cells contribute to liver regeneration through galectins in hepatic stem cell niche Stem Cell Res Ther, 11(1):425.
PMID: 32993816
Species: Mouse

Applications: Western Blot

MS Tsai et al. (2018), Galectin-1 Restricts Vascular Smooth Muscle Cell Motility Via Modulating Adhesion Force and Focal Adhesion Dynamics Sci Rep, 8(1):11497.
PMID: 30065303
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

N Yang et al. (2017), Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy Exp. Eye Res, 0(0).
PMID: 28257831
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Scheffer L et al. (2014), Mechanism of Ca(2)(+)-triggered ESCRT assembly and regulation of cell membrane repair. Nat Commun, 5(0):5646.
PMID: 25534348
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Baker G et al. (2014), Natural killer cells eradicate galectin-1-deficient glioma in the absence of adaptive immunity. Cancer Res, 74(18):5079-90.
PMID: 25038230
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Szebeni G et al. (2012), Identification of galectin-1 as a critical factor in function of mouse mesenchymal stromal cell-mediated tumor promotion. PLoS ONE, 7(7):e41372.
PMID: 22844466
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

Qu WS et al. (2011), Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia. J. Neurochem., 116(2):217-26.
PMID: 21054390
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

(2011), Impaired spatial and contextual memory formation in galectin-1 deficient mice. Mol Brain, 4(0):33.
PMID: 21884595
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Iqbal AJ et al. (2011), Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect. Am. J. Pathol., 178(3):1201-9.
PMID: 21356371
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Shimizu M et al. (2009), Expression of galectin-1, a new component of slit diaphragm, is altered in minimal change nephrotic syndrome. Lab. Invest., 89(2):178-95.
PMID: 19079321
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Plachta N et al. (2007), Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells. Nat. Neurosci., 10(6):712-9.
PMID: 17486104
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Silva-Monteiro E et al. (2007), Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection. Am. J. Pathol., 170(2):546-56.
PMID: 17255323
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Immunoprecipitation

Endharti AT et al. (2005), Galectin-1 supports survival of naive T cells without promoting cell proliferation. Eur. J. Immunol., 35(1):86-97.
PMID: 15593120

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Customer Reviews for Mouse Galectin-1 Antibody (5)

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Mouse Galectin-1 Antibody

Name: Anonymous

Application: WB

Sample Tested: U-87 MG human glioblastoma/astrocytoma cell line

Species: Mouse

Verified Customer | Posted 08/17/2016

1:2000 dilution in TBS-Tween 20 + 5% BSA
Mouse Galectin‑1 Antibody

Name: Anonymous

Application: WB

Sample Tested: Cell Lysates

Species: Human

Verified Customer | Posted 10/26/2015

The antibody is very specific at 1:1000 and also at 1:2500 dilutions. Specificity: SpecificSensitivity: SensitiveBuffer: BSADilution: 1:1000
Mouse Galectin‑1 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Colon tissue

Species: Mouse

Verified Customer | Posted 10/26/2015

The antibody specifically detects the stromal galectin-1 in normal tissues. Specificity: SpecificSensitivity: SensitiveBuffer: PBSDilution: 1:1000
Mouse Galectin‑1 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Human fibroblast

Species: Human

Verified Customer | Posted 10/26/2015

These are HFF-2 cells that show the staining for this antibody. Specificity: SpecificSensitivity: SensitiveBuffer: PBS`Dilution: 1:500
Mouse Galectin‑1 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Cancer Cells

Species: Human

Verified Customer | Posted 10/26/2015

Very specific in detection of galectin-1 in cancer cells. Specificity: SpecificSensitivity: SensitiveBuffer: PBSDilution: 1:1000

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Mouse Galectin-1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse Galectin-1 Antibody

Mouse Galectin-1 Sandwich Immunoassay

Scientific Data Examples for Mouse Galectin-1 Antibody

Immunohistochemistry

Intracellular Staining by Flow Cytometry

Simple Western

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Galectin-1

Citations for Mouse Galectin-1 Antibody (16)

Customer Reviews for Mouse Galectin-1 Antibody (5)

Customer Images

Mouse Galectin-1 Antibody

Mouse Galectin‑1 Antibody

Mouse Galectin‑1 Antibody

Mouse Galectin‑1 Antibody

Mouse Galectin‑1 Antibody

Product Documents for Mouse Galectin-1 Antibody

Product Datasheets

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