Mouse Dectin-1/CLEC7A Antibody

Name: Mouse Dectin-1/CLEC7A Antibody
Brand: R&D Systems
SKU: MAB1756
Rating: 3 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1756

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(2)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse Dectin-1 Phe69-Leu244 Accession # Q6QLQ4
Applications:	Western Blot
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2B Clone # 218838
Specificity:	Detects mouse Dectin-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Dectin‑1, rmDectin-2 alpha, or rhDLEC.

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Applications for Mouse Dectin-1/CLEC7A Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse Dectin‑1/CLEC7A (Catalog # 1756-DC)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25% - 34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 ‑ 10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 ‑ 13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

Item	Value
References	Kanazawa, N. (2007) J. Dermatol. Sci. 45:77. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. Willment, J.A. et al. (2003) J. Immunol. 171:4569. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771. Gantner, B.N. et al. (2005) EMBO J. 24:1277. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119. Yadav, M. and J.S. Schorey (2006) Blood 108:3168. Suram, S. et al. (2006) J. Biol. Chem. 281:5506. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154. Saijo, S. et al. (2007) Nat. Immunol. 8:39. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs	64581 (Human); 56644 (Mouse)
Alternate Names	BGR, Beta-glucan receptor, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12, C-type lectin domain family 7 member A, C-type lectin domain family 7, member A, C-type lectin superfamily member 12, CD369, CLEC7A, CLECSF12, CLECSF12DC-associated C-type lectin 1, DECTIN1CANDF4, Dectin-1, Dectin1, Dendritic cell-associated C-type lectin 1, dendritic cell-associated C-type lectin-1, hDectin-1, lectin-like receptor 1

Citations for Mouse Dectin-1/CLEC7A Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Dectin-1/CLEC7A Antibody include:

Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Li X et al. (2015), Protein-tyrosine phosphatase Shp2 positively regulates macrophage oxidative burst. J Biol Chem, 290(7):3894-909.
PMID: 25538234

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Customer Reviews for Mouse Dectin-1/CLEC7A Antibody (2)

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Mouse Dectin-1/CLEC7A Antibody

Name: Anonymous

Application: Flow

Sample Tested: 293T human embryonic kidney cell line

Species: Mouse

Verified Customer | Posted 11/28/2020

HEK cells were transfected with mouse Dectin1 isoform 2 and stained with the Rat anti-mouse Dectin1 clone 218838 at a concentration of 0.005 nM to 300 nM. The 218838 antibody was detected using a secondary DyLight 405 AffiniPure Mouse Anti-Rat IgG, F(ab')₂ fragment specific antibody. The binding affinity was calculated at EC50 = 64 nM. Binding was observed only in the higher concentrations of the antibody.

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Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
Mouse Dectin-1/CLEC7A Antibody

Name: Anonymous

Application: Flow

Sample Tested: Transfected HEK cells overexpressing mouse Dectin-1b

Species: Mouse

Verified Customer | Posted 10/13/2020

Detection of stalkless mouse Dectin1 isoform B in transfected HEK cells by Flow Cytometry. HEK cells overexpressing the murine Dectin1B isoform (stalkless) were stained with the Rat anti-mouse Dectin1 clone 218838 at 100 nM or the Rat IgG2B isotype control. Binding was detected with a secondary a-Rat IgG (H+L) Pacific blue antibody.

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Mouse Dectin-1/CLEC7A Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Mouse Dectin-1/CLEC7A Antibody

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Dectin-1/CLEC7A

Citations for Mouse Dectin-1/CLEC7A Antibody (1)

Customer Reviews for Mouse Dectin-1/CLEC7A Antibody (2)

Customer Images

Mouse Dectin-1/CLEC7A Antibody

Mouse Dectin-1/CLEC7A Antibody

Product Documents for Mouse Dectin-1/CLEC7A Antibody

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