Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB452
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Bioassay, Blocking, ELISA Capture, ELISA Development, ELISA Development (Capture), In vivo assay
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 40605
Product Specifications
Immunogen
E. coli-derived recombinant mouse CXCL2/GRO beta/MIP-2/CINC-3
Ala28-Asn100
Accession # P10889
Ala28-Asn100
Accession # P10889
Specificity
Detects mouse CXCL2 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) CXCL2/GRO beta, rhCXCL3/GRO gamma, or recombinant mouse CXCL1/KC.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
Chemotaxis Induced by CXCL2/MIP-2 and Neutralization by Mouse CXCL2/MIP-2 Antibody.
Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP-2 Monoclonal Antibody (Catalog # MAB452). The ND50 is typically 1-4 µg/mL.
Detection of CXCL2/GRO beta/MIP-2/CINC-3 by Immunohistochemistry
G‐CSF and MIP‐2 in exosomes were the main protection effectors in testis radiation protection. A, On day 7 after neutralizing antibody administration and 4 Gy irradiation, mice testis was isolated and subjected to H&E staining. B, Clonal formation assay was used to determine GC‐1 cell viability after exosomes treatment and irradiation exposure with or without neutralizing antibody. Data were presented as mean ± SD (n = 3). *P < .05. **P < .01, ***P < .001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32135028), licensed under a CC-BY license. Not internally tested by R&D Systems.
Mouse CXCL2 / GRO beta / MIP-2 / CINC-3 ELISA Standard Curve
Recombinant Mouse CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 452-M2) was serially diluted and captured by Rat Anti-Mouse CXCL2/GRO beta/MIP-2/CINC-3 Monoclonal Antibody (Catalog # MAB452) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 452-M2)
under non-reducing conditions only
Sample: Recombinant Mouse CXCL2/GRO beta/MIP-2/CINC-3 (Catalog # 452-M2)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize CXCL2/GRO beta/MIP-2/CINC-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta/MIP-2/CINC-3.
Mouse CXCL2/MIP-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4 using MAB452 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL2/GRO beta/MIP-2/CINC-3
Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.
MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.
Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.
Alternate Names
CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2
Gene Symbol
CXCL2
UniProt
Additional CXCL2/GRO beta/MIP-2/CINC-3 Products
Product Documents for Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
Product Specific Notices for Mouse CXCL2/GRO beta/MIP-2/CINC-3 Antibody
For research use only
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