Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody

Name: Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF470
Rating: 4 (1 reviews)

Catalog # BAF470 | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (5)

Product Datasheet / COA / SDS

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Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

ELISA Detection (Matched Antibody Pair), Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody

Immunogen

E. coli-derived recombinant mouse CXCL13/BLC (R&D Systems, Catalog # 470-BC)
Ile22-Ala109
Accession # Q3U1E8

Specificity

Detects mouse CXCL13 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human CXCL13, recombinant mouse (rm) I-TAC, rmMIG, and rmSDF-1 alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC)

Mouse CXCL13/BLC/BCA-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse CXCL13/BLC/BCA‑1 Antibody (Catalog # MAB470R)
Standard: Recombinant Mouse CXCL13/BLC/BCA-1 Protein (Catalog # 470-BC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using BAF470 in the following applications:

Immunohistochemistry-Frozen (1 Review)

Published Applications

Read 5 publications using BAF470 in the following applications:

IHC (3 Publications)
IHC-Fr (1 Publication)
Luminex Development (1 Publication)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

Gunn, M.D. et al. (1998) Nature, 391:799.
Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
Forster, R. et al. (1996) Cell 87:1037.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol

CXCL13

UniProt

Q3U1E8

Product Documents for Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody

For research use only

Citations
Reviews

Citations for Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody (5)

Showing 1 - 5 of 5 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

HW Cheng et al. (2022-04-19), Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function Nature Communications, 2022-013(1):2027.
PMID: 35440118
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Nakagawa R et al. (2013-03-04), Peyer's patch inducer cells play a leading role in the formation of B and T cell zone architecture. J Immunol, 2013-0190(7):3309-18.
PMID: 23460741
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Baptista A et al. (2012-09-19), Colonic patch and colonic SILT development are independent and differentially regulated events. Mucosal Immunol, 2012-06(3):511-21.
PMID: 22990625
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development

Zhang M et al. (2012-01-25), Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-058(2):169-77.
PMID: 22277799
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Katakai T et al. (2008-11-01), Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs. J. Immunol., 2008-1181(9):6189-200.
PMID: 18941209

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Customer Reviews for Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody (1)

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