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Mouse CXCL13/BLC/BCA‑1 Antibody

Catalog # MAB470R | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Monoclonal Antibody.
Catalog #
Size / Price

Key Product Details

Species Reactivity



ELISA Capture (Matched Antibody Pair), Neutralization



Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # 143614R

Product Summary for Mouse CXCL13/BLC/BCA‑1 Antibody


E. coli-derived recombinant mouse CXCL13
Accession # O55038


Detects mouse CXCL13 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL13, rmCXCL11/I‑TAC, rmCXCL9/MIG, or rm CXCL12/SDF‑1 alpha.







Scientific Data Images for Mouse CXCL13/BLC/BCA‑1 Antibody

Chemotaxis Induced by CXCL13/BLC/BCA-1 and Neutralization by Mouse CXCL13/BLC/BCA-1 Antibody

Chemotaxis Induced by CXCL13/BLC/BCA-1 and Neutralization by Mouse CXCL13/BLC/BCA-1 Antibody

Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA-1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Recombinant Mouse CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB470R). The ND50 is typically 12-30 µg/mL.

Applications for Mouse CXCL13/BLC/BCA‑1 Antibody

Recommended Usage


Measured by its ability to neutralize CXCL13/BLC/BCA-1 induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 12-30 μg/mL in the presence of 4 μg/mL Recombinant Mouse CXCL13/BLC/BCA-1.

Mouse CXCL13/BLC/BCA-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody (Catalog # BAF470)
  • Standard: Recombinant Mouse CXCL13/BLC/BCA-1 Protein (Catalog # 470-BC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using MAB470R in the following applications:

Formulation, Preparation and Storage


Protein A or G purified from cell culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.


Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC) and BCA-1, is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse CXCL13 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse CXCL13 shares 64% amino acid sequence similarity with the human protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized CXCL13 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for CXCL13. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.


  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

Alternate Names


Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol



Product Documents for Mouse CXCL13/BLC/BCA‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CXCL13/BLC/BCA‑1 Antibody

For research use only

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