Mouse CXCL13/BLC/BCA‑1 Antibody

Name: Mouse CXCL13/BLC/BCA‑1 Antibody
Brand: R&D Systems
SKU: AF470
Rating: 4 (2 reviews)

Catalog # AF470 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (37)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse CXCL13/BLC/BCA‑1 Antibody

Immunogen

E. coli-derived recombinant mouse CXCL13/BLC/BCA‑1
Ile22-Ala109
Accession # Q3U1E8

Specificity

Detects mouse CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CXCL13/BLC/BCA‑1 Antibody

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody.

Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin staining. Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA-1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL13/ BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470). The ND₅₀ is typically 20-50 µg/mL.

CXCL13/BLC/BCA‑1 in Mouse Intestine.

CXCL13/BLC/BCA-1 was detected in perfusion fixed frozen sections of mouse intestine (Peyer's patch) using Goat Anti-Mouse CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse CXCL13/BLC/BCA‑1 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine (Peyer's patch)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC)

Neutralization

Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND₅₀) is typically 20-50 µg/mL in the presence of 4 µg/mL Recombinant Mouse CXCL13/BLC/BCA‑1.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using AF470 in the following applications:

Immunofluorescence (1 Review)
Immunohistochemistry-Frozen (1 Review)

Published Applications

Read 37 publications using AF470 in the following applications:

Bioassay (1 Publication)
Flow Cytometry (2 Publications)
ICC (1 Publication)
IHC (13 Publications)
IHC-F (1 Publication)
IHC-Fr (10 Publications)
IHC-P (6 Publications)
In Vivo (1 Publication)
Migration Assay (1 Publication)
Neutralization (1 Publication)
Western Blot (3 Publications)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

Gunn, M.D. et al. (1998) Nature, 391:799.
Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
Forster, R. et al. (1996) Cell 87:1037.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol

CXCL13

UniProt

Q3U1E8

Product Documents for Mouse CXCL13/BLC/BCA‑1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Mouse CXCL13/BLC/BCA‑1 Antibody

For research use only

Citations
Reviews

Citations for Mouse CXCL13/BLC/BCA‑1 Antibody (37)

Showing 1 - 10 of 37 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CXCL13/BLC/BCA‑1 Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

MC Trolese et al. (2020-11-09), CXCL13/CXCR5 signalling is pivotal to preserve motor neurons in amyotrophic lateral sclerosis EBioMedicine, 2020-162(0):103097.
PMID: 33161233
Species: Mouse
Sample Types: Whole Cells
Applications: Migration Assay

QF Zhang et al. (2020-08-25), CDK4/6 inhibition promotes immune infiltration in ovarian cancer and synergizes with PD-1 blockade in a B cell-dependent manner Theranostics, 2020-010(23):10619-10633.
PMID: 32929370
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

K Streich et al. (2020-04-29), Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes J Adv Res, 2020-024(0):291-300.
PMID: 32405435
Species: Mouse
Applications: In Vivo

N Muthalagu et al. (2020-03-21), Repression of the Type I Interferon pathway underlies MYC & KRAS-dependent evasion of NK & B cells in Pancreatic Ductal Adenocarcinoma Cancer Discov, 2020-00(0).
PMID: 32200350
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

X Jia et al. (2019-11-25), Foliate Lymphoid Aggregates as Novel Forms of Serous Lymphocyte Entry Sites of Peritoneal B Cells and High-Grade B Cell Lymphomas J. Immunol., 2019-10(0).
PMID: 31767783
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-F

LK Dubey et al. (2019-05-21), IL-4R?-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells Cell Rep, 2019-027(8):2442-2458.e5.
PMID: 31116987
Species: Mouse
Sample Types: Whole Cells

AC Billi et al. (2019-04-18), The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity JCI Insight, 2019-04(8).
PMID: 30996136
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

HW Cheng et al. (2019-04-15), Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp Nat Commun, 2019-010(1):1739.
PMID: 30988302
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

M Kim et al. (2018-11-06), Human Adipose Tissue-Derived Mesenchymal Stem Cells Attenuate Atopic Dermatitis by Regulating the Expression of MIP-2, miR-122a-SOCS1 Axis, and Th1/Th2 Responses Front Pharmacol, 2018-19(0):1175.
PMID: 30459600
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

M Gallotta et al. (2018-06-26), Inhaled TLR9 Agonist Renders Lung Tumors Permissive to PD-1 Blockade by Promoting Optimal CD4+ and CD8+ T cell Interplay Cancer Res., 2018-00(0).
PMID: 29945961
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

IK Sundar et al. (2018-04-09), Genetic ablation of histone deacetylase 2 leads to lung cellular senescence and lymphoid follicle formation in COPD/emphysema FASEB J., 2018-00(0):fj201701518R.
PMID: 29630406
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

G Porras et al. (2018-03-28), Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice Int. J. Biochem. Cell Biol., 2018-099(0):72-79.
PMID: 29604348
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

DG Hill et al. (2018-02-19), Hyperactive gp130/STAT3-driven gastric tumourigenesis promotes submucosal tertiary lymphoid structure development Int. J. Cancer, 2018-00(0).
PMID: 29417587
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

DT Mzinza et al. (2018-02-12), Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice Cell. Mol. Immunol., 2018-00(0).
PMID: 29429996
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

W Alturaiki et al. (2018-02-01), Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection Cytokine, 2018-00(0).
PMID: 29396045
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

D Respatika et al. (2017-08-10), Role of SIRP? in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen Kobe J Med Sci, 2017-063(1):E22-E29.
PMID: 29434170
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

VM Turner et al. (2017-05-04), Ageing adversely affects the migration and function of marginal zone B cells Immunology, 2017-0151(3):349-362.
PMID: 28369800
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

T Eddens et al. (2017-03-28), Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity Cell Rep, 2017-018(13):3078-3090.
PMID: 28355561
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

(2016-11-28), Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy J Neuroinflammation, 2016-113(1):298.
PMID: 27894304
Species: Mouse
Sample Types: Cell Lysates,Whole Tissue
Applications: Western Blot,IHC-P

(2015-12-29), Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia Cancer Discov, 2015-16(3):256-69.
PMID: 26715642
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Klimatcheva E et al. (2015-02-12), CXCL13 antibody for the treatment of autoimmune disorders. BMC Immunol, 2015-016(0):6.
PMID: 25879435
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Buettner M et al. (2014-11-05), Stromal cells as trend-setters for cells migrating into the lymph node. Mucosal Immunol, 2014-18(3):640-9.
PMID: 25354321
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Fleige H et al. (2014-03-24), IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs. J Exp Med, 2014-0211(4):643-51.
PMID: 24663215
Species: Mouse
Sample Types: In Vivo,Cell Lysates,Whole Tissue
Applications: Western Blot,IHC,Neutralization

Yamamoto et al. (2014-03-19), Anti-CXCL13 antibody can inhibit the formation of gastric lymphoid follicles induced by Helicobacter infection. Mucosal Immunol, 2014-07(5):1244-54.
PMID: 24646940
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Benezech C et al. (2014-02-14), CLEC-2 is required for development and maintenance of lymph nodes. Blood, 2014-0123(20):3200-7.
PMID: 24532804
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Monson N et al. (2014-01-31), Repetitive hypoxic preconditioning induces an immunosuppressed B cell phenotype during endogenous protection from stroke. J Neuroinflammation, 2014-011(0):22.
PMID: 24485041
Species: Mouse
Sample Types: Whole Cells,Whole Tissue
Applications: IHC,ICC

Mionnet C et al. (2013-10-01), Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles. PLoS Biol, 2013-111(10):e1001672.
PMID: 24130458
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Kramer J et al. (2013-07-31), CXCL13 is elevated in Sjogren's syndrome in mice and humans and is implicated in disease pathogenesis. J Leukoc Biol, 2013-094(5):1079-89.
PMID: 23904442
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Khader SA et al. (2011-10-14), IL-23 is required for long-term control of Mycobacterium tuberculosis and B cell follicle formation in the infected lung. J. Immunol., 2011-1187(10):5402-7.
PMID: 22003199
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Suzuki K et al. (2010-07-23), The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut. Immunity, 2010-033(1):71-83.
PMID: 20643338
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Eberlein J et al. (2010-02-08), Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-0120(3):907-23.
PMID: 20197626
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

St John AL et al. (2009-10-25), Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines. Nat. Med., 2009-115(11):1259-65.
PMID: 19855398
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Demoor T et al. (2009-01-22), Role of lymphotoxin-alpha in cigarette smoke-induced inflammation and lymphoid neogenesis. Eur. Respir. J., 2009-034(2):405-16.
PMID: 19164352
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Grabner R et al. (2009-01-12), Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice. J. Exp. Med., 2009-0206(1):233-48.
PMID: 19139167
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Martin AP et al. (2006-11-15), The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo. J. Immunol., 2006-1177(10):7296-302.
PMID: 17082648
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Ha SA et al. (2006-10-23), Regulation of B1 cell migration by signals through Toll-like receptors. J. Exp. Med., 2006-1203(11):2541-50.
PMID: 17060475
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Columba-Cabezas S et al. (2006-07-25), Suppression of established experimental autoimmune encephalomyelitis and formation of meningeal lymphoid follicles by lymphotoxin beta receptor-Ig fusion protein. J. Neuroimmunol., 2006-0179(1):76-86.
PMID: 16870269

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