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Mouse CD68/SR‑D1 Antibody

Catalog # MAB101141 | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Monoclonal Antibody.

Key Product Details

Species Reactivity



CyTOF-ready, Immunohistochemistry, Intracellular Staining by Flow Cytometry



Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2449D

Product Summary for Mouse CD68/SR‑D1 Antibody


Chinese Hamster Ovary cell line, CHO-derived mouse CD68
Accession # P31996


Detects mouse CD68 in direct ELISAs.







Scientific Data Images for Mouse CD68/SR‑D1 Antibody

Detection of CD68 antibody in Raw264.7 Mouse Cell line antibody by Flow Cytometry.

Detection of CD68 in Raw264.7 Mouse Cell line by Flow Cytometry.

Raw264.7 mouse cell line was stained with Rabbit Anti-Mouse CD68 Monoclonal Antibody (Catalog # MAB101141, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1x) (Catalog # FC004) and permeabilized with ice-cold methanol.
CD68/SR-D1 antibody in Mouse Liver by Immunohistochemistry (IHC-P).

CD68/SR‑D1 in Mouse Liver.

CD68/SR-D1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Mouse CD68/SR-D1 Monoclonal Antibody (Catalog # MAB101141) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse CD68/SR‑D1 Antibody

Recommended Usage


Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.


3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse liver

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: RAW264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with ice-cold methanol
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using MAB101141 in the following applications:

Formulation, Preparation, and Storage


Protein A or G purified from cell culture supernatant


Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD68/SR-D1

Macrosialin (CD68) is a macrophage-restricted glycosylated transmembrane protein.  It belongs to the lamp family and carries out specialized functions in dedicated phagocytic cells.  Macrosialin binds to tissue- and organ-specific lectins to allow homing of macrophages to specific targets.  Its presence in macrophages is useful in diagnosing conditions related to proliferation or abnormality of these cells.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Entrez Gene IDs

968 (Human); 12514 (Mouse); 287435 (Rat)

Gene Symbol



Additional CD68/SR-D1 Products

Product Documents for Mouse CD68/SR‑D1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD68/SR‑D1 Antibody

For research use only


Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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