Mouse CD3 Antibody

Detection of CD3 in Mouse Thymus.

CD3 was detected in perfusion fixed paraffin-embedded sections of Mouse Thymus using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence

(A) T cells (CD3+ cells) were increased at 2 and 7 days postinjury (DPI) compared to 14 DPI (*, P < 0.05) in injured muscles of both wild‐type (WT) and CXCL10 knockout (KO) mice. (B) A representative micrograph of a T‐cell stain (Red = CD3, Blue = DNA) in a WT mouse at 7 DPI. Arrows point out examples of CD3‐positive cells. (C) CXCR3‐positive cells were increased at 2 and 7 DPI compared to 14 DPI (*P < 0.05) in both genotypes. (D) A representative micrograph of CXCR3 (green) stain in a WT mouse at 7 DPI. Arrows mark examples of CXCR3‐positive cells. (E) Approximately 50% of the T cells (CD3 cells) were CXCR3 positive (the CXCL10 receptor). No differences were observed between genotypes or between 2 and 7 DPI. (F) A merged image of panel B and D. The arrow points out an example a cell positive for both CD3 and CXCR3. The circle shows a CD3‐positive, CXCR3‐negative cell. Data are mean ± SD. Scale bar is 100 μm. n = 4–6 per experimental condition. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29696819), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence

(A) T cells (CD3+ cells) were increased at 2 and 7 days postinjury (DPI) compared to 14 DPI (*, P < 0.05) in injured muscles of both wild‐type (WT) and CXCL10 knockout (KO) mice. (B) A representative micrograph of a T‐cell stain (Red = CD3, Blue = DNA) in a WT mouse at 7 DPI. Arrows point out examples of CD3‐positive cells. (C) CXCR3‐positive cells were increased at 2 and 7 DPI compared to 14 DPI (*P < 0.05) in both genotypes. (D) A representative micrograph of CXCR3 (green) stain in a WT mouse at 7 DPI. Arrows mark examples of CXCR3‐positive cells. (E) Approximately 50% of the T cells (CD3 cells) were CXCR3 positive (the CXCL10 receptor). No differences were observed between genotypes or between 2 and 7 DPI. (F) A merged image of panel B and D. The arrow points out an example a cell positive for both CD3 and CXCR3. The circle shows a CD3‐positive, CXCR3‐negative cell. Data are mean ± SD. Scale bar is 100 μm. n = 4–6 per experimental condition. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29696819), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse CD3 by Immunohistochemistry

Peripheral mCRP‐induced neuroinflammation, extravasation of T lymphocytes&AD pathology traits in the ApoE4 brain. (a) We then investigated if mCRP‐induced cerebrovascular damage can migrate peripheral immune cells into the brain. Double immunostaining of CD8 (red), CD3 (green),&double‐positive cells (yellow) was performed to study the transcytosis of T lymphocytes in the cortex after i.p. treatment with PBS (left columns) versus mCRP (right columns) in WT&different ApoE knock‐in mice. Quantification of CD8+ T lymphocytes&CD8+/CD3+ T lymphocytes in the cortex&the comparisons between PBS versus mCRP treatment in each genotype was conducted. mCRP significantly increased the number of T lymphocytes only in the WT (p = 0.05)&ApoE4 (p = 0.001) mice. n = 7–8 in each condition. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34687487), licensed under a CC-BY license. Not internally tested by R&D Systems.