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Mouse CD3 Antibody
Catalog # MAB4841 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Mouse
Applications
Complement-dependent Cytotoxicity, CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, T Cell Stimulation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 17A2
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Product Summary for Mouse CD3 Antibody
Immunogen
T cell hybridoma D1
Specificity
Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse CD3 Antibody
CD3 in Mouse Splenocytes.
CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD3 in Mouse Brain.
CD3 was detected in perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) using 10 µg/mL Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of CD3 in Mouse Thymus.
CD3 was detected in perfusion fixed paraffin-embedded sections of Mouse Thymus using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse CD3 Antibody
Application
Recommended Usage
Complement-dependent Cytotoxicity
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113 and Wu, L. et al. (1991) J. Exp. Med. 174:1617.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) and perfusion fixed paraffin-embedded sections of Mouse Thymus
Sample: Perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) and perfusion fixed paraffin-embedded sections of Mouse Thymus
Immunoprecipitation
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
T Cell Stimulation
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 5 using MAB4841 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3
References
- Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
Alternate Names
CD3, CD3E, CD_antigen: CD3e, CD3 antigen, delta subunit, CD3-DELTA, CD3-epsilon, CD3-GAMMA, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3G, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T3DOKT3, delta chain, T3E, TCRE
Gene Symbol
CD3E
Product Documents for Mouse CD3 Antibody
Product Specific Notices for Mouse CD3 Antibody
For research use only
Citations for Mouse CD3 Antibody (29)
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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD3 Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationO Anannya et al. (2023-04-03), ITK signaling regulates a switch between T helper 17 and T regulatory cell lineages via a calcium-mediated pathway bioRxiv : the preprint server for biology, 2023-00(0).
PMID: 37066370 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCJH Cha et al. (2022-03-10), Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer The Journal of Biological Chemistry, 2022-00(0):101817.
PMID: 35278434 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IFZ Zhang et al. (2021-10-23), Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease? Aging Cell, 2021-10(0):e13501.
PMID: 34687487 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationJ Zhou et al. (2021-05-12), The YAP/HIF-1&alpha/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation Cell & bioscience, 2021-011(1):84.
PMID: 33980319 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCD Li et al. (2021-04-21), Itgb3-integrin-deficient mice may not be a sufficient�model for patients with Glanzmann thrombasthenia Molecular Medicine Reports, 2021-023(6).
PMID: 33880575 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCY Kim et al. (2020-12-09), Hepatocyte cannabinoid 1 receptor nullification alleviates toxin-induced liver damage via NF-&kappaB signaling Cell Death & Disease, 2020-111(12):1044.
PMID: 33298885 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayC Alvarez et al. (2020-11-04), Regulatory T cell phenotype and anti-osteoclastogenic function in experimental periodontitis Sci Rep, 2020-110(1):19018.
PMID: 33149125 -
Species: Mouse
Sample Types: In Vivo,Whole Cells,Whole Tissue
Applications: Neutralization,IHC,Flow Cytometry,ICCJ Chida et al. (2020-08-26), Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice PLoS Pathog., 2020-016(8):e1008823.
PMID: 32845931 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PYP Xu et al. (2019-07-16), Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy J. Clin. Invest., 2019-0130(0).
PMID: 31310587 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PL Wen et al. (2019-07-10), Contributions of T cell dysfunction to the resistance against anti-PD-1 therapy in oral carcinogenesis J. Exp. Clin. Cancer Res., 2019-038(1):299.
PMID: 31291983 -
Species: Mouse
Sample Types: Whole Cells
Applications: ActivationJ Wu et al. (2018-08-07), Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3+ T Cell through Modulation of CD11b+ Cell Function J Immunol Res, 2018-02018(0):1605341.
PMID: 30159338 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCMR Deyhle et al. (2018-04-01), CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration Physiol Rep, 2018-06(8):e13689.
PMID: 29696819 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCP Chauhan et al. (2018-03-05), Nitrosative damage during retrovirus infection-induced neuropathic pain J Neuroinflammation, 2018-015(1):66.
PMID: 29506535 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayW Lei et al. (2018-01-18), Crucial role of OX40/OX40L signaling in a murine model of asthma Mol Med Rep, 2018-00(0).
PMID: 29344664 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCS Swirski et al. (2018-01-01), A Novel C-Terminal Mutation in Gsdma3 (C+/H-) Leads to Alopecia and Corneal Inflammatory Response in Mice Invest. Ophthalmol. Vis. Sci., 2018-059(1):561-571.
PMID: 29372254 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayX Fu et al. (2017-09-19), Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression Mediators Inflamm., 2017-02017(0):5706152.
PMID: 29056830 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayR Fischer et al. (2017-07-26), Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2 Sci Rep, 2017-07(1):6607.
PMID: 28747780 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayW Pan et al. (2017-07-21), The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells Parasit Vectors, 2017-010(1):348.
PMID: 28732522 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCPG Makker et al. (2017-01-26), Characterisation of Immune and Neuroinflammatory Changes Associated with Chemotherapy-Induced Peripheral Neuropathy PLoS ONE, 2017-012(1):e0170814.
PMID: 28125674 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICC(2016-12-15), Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology Alzheimers Res Ther, 2016-18(1):54.
PMID: 27974048 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayDavid A Hildeman (2016-11-16), Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCR? Repertoire J. Immunol., 2016-10(0).
PMID: 27852740 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCOuyang M et al. (2016-02-01), Metronomic Doses of Temozolomide Enhance the Efficacy of Carbon Nanotube CpG Immunotherapy in an Invasive Glioma Model. PLoS ONE, 2016-011(2):e0148139.
PMID: 26829221 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayFan et al. (2014-09-24), Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif. PLoS ONE, 2014-09(9):e108253.
PMID: 25250958 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCAhn et al. (2013-12-17), Ninjurin1 deficiency attenuates susceptibility of experimental autoimmune encephalomyelitis in mice. J Biol Chem, 2013-1289(6):3328-38.
PMID: 24347169 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayVang et al. (2013-12-15), Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac. Biochem J, 2013-1456(3):463-73.
PMID: 24007532 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCNakamura et al. (2013-12-09), LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis. J Clin Invest, 2013-1124(1):385-97.
PMID: 24316976 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayXu C et al. (2013-11-29), TGF-beta promotes immune responses in the presence of mesenchymal stem cells. J Immunol, 2013-1192(1):103-9.
PMID: 24293629 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PDe Palma AM et al. (2008-10-16), Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis. Virology, 2008-1382(1):20-7.
PMID: 18929380 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayNguyen P et al. (2003-08-28), Identification of a murine CD28 dileucine motif that suppresses single-chain chimeric T-cell receptor expression and function. Blood, 2003-0102(13):4320-5.
PMID: 12946999
There are no citations that match your criteria.
Customer Reviews for Mouse CD3 Antibody (4)
5 out of 5
4 customer
ratings
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Application: ImmunohistochemistrySample Tested: Spleen tissueSpecies: MouseVerified Customer | Posted 02/17/2022
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Application: ImmunohistochemistrySample Tested: Spleen tissueSpecies: MouseVerified Customer | Posted 08/13/2021
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Paraffined tumor tissue from HCC xenograftSpecies: MouseVerified Customer | Posted 06/07/2018Green fluorescent is CD3 in mice tumors.
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Adult heartSpecies: MouseVerified Customer | Posted 05/16/2017Permeabilisation - 30mins at RT Block -1% BSA - 30mins at RT Primary Ab - diluted in block - 1 in 25 - for O/N at 4'C Secondary ab - diluted in block - 2hrs at RT
There are no reviews that match your criteria.
FAQs for Mouse CD3 Antibody
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Q: What is the optimal working concentration of the antibody?
A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.
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