Mouse CD3 Antibody

Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence

(A) T cells (CD3+ cells) were increased at 2 and 7 days postinjury (DPI) compared to 14 DPI (*, P < 0.05) in injured muscles of both wild‐type (WT) and CXCL10 knockout (KO) mice. (B) A representative micrograph of a T‐cell stain (Red = CD3, Blue = DNA) in a WT mouse at 7 DPI. Arrows point out examples of CD3‐positive cells. (C) CXCR3‐positive cells were increased at 2 and 7 DPI compared to 14 DPI (*P < 0.05) in both genotypes. (D) A representative micrograph of CXCR3 (green) stain in a WT mouse at 7 DPI. Arrows mark examples of CXCR3‐positive cells. (E) Approximately 50% of the T cells (CD3 cells) were CXCR3 positive (the CXCL10 receptor). No differences were observed between genotypes or between 2 and 7 DPI. (F) A merged image of panel B and D. The arrow points out an example a cell positive for both CD3 and CXCR3. The circle shows a CD3‐positive, CXCR3‐negative cell. Data are mean ± SD. Scale bar is 100 μm. n = 4–6 per experimental condition. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29696819), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence

(A) T cells (CD3+ cells) were increased at 2 and 7 days postinjury (DPI) compared to 14 DPI (*, P < 0.05) in injured muscles of both wild‐type (WT) and CXCL10 knockout (KO) mice. (B) A representative micrograph of a T‐cell stain (Red = CD3, Blue = DNA) in a WT mouse at 7 DPI. Arrows point out examples of CD3‐positive cells. (C) CXCR3‐positive cells were increased at 2 and 7 DPI compared to 14 DPI (*P < 0.05) in both genotypes. (D) A representative micrograph of CXCR3 (green) stain in a WT mouse at 7 DPI. Arrows mark examples of CXCR3‐positive cells. (E) Approximately 50% of the T cells (CD3 cells) were CXCR3 positive (the CXCL10 receptor). No differences were observed between genotypes or between 2 and 7 DPI. (F) A merged image of panel B and D. The arrow points out an example a cell positive for both CD3 and CXCR3. The circle shows a CD3‐positive, CXCR3‐negative cell. Data are mean ± SD. Scale bar is 100 μm. n = 4–6 per experimental condition. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29696819), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse CD3 by Immunohistochemistry

Peripheral mCRP‐induced neuroinflammation, extravasation of T lymphocytes&AD pathology traits in the ApoE4 brain. (a) We then investigated if mCRP‐induced cerebrovascular damage can migrate peripheral immune cells into the brain. Double immunostaining of CD8 (red), CD3 (green),&double‐positive cells (yellow) was performed to study the transcytosis of T lymphocytes in the cortex after i.p. treatment with PBS (left columns) versus mCRP (right columns) in WT&different ApoE knock‐in mice. Quantification of CD8+ T lymphocytes&CD8+/CD3+ T lymphocytes in the cortex&the comparisons between PBS versus mCRP treatment in each genotype was conducted. mCRP significantly increased the number of T lymphocytes only in the WT (p = 0.05)&ApoE4 (p = 0.001) mice. n = 7–8 in each condition. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34687487), licensed under a CC-BY license. Not internally tested by R&D Systems.