Mouse B220/CD45R PE-conjugated Antibody

Name: Mouse B220/CD45R PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB1217P
Price: 314 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB1217P

Citations (1)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Abelson mouse leukemia virus-induced pre-B tumor cells
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Rat IgG_2A Clone # RA3-6B2
Specificity:	Detects mouse B220/CD45R.

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Applications for Mouse B220/CD45R PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse B220/CD45R PE-conjugated Antibody

	Flow Cytometry Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) or (B) Rat IgG_2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

Item	Value
References	Coffman, R.L. (1982) Immuno. Rev. 69:5. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286. Allman, D.M. et al. (1992) J. Immunol. 149:2533. Asensi, V.K. et al. (1989) Immunology 68:204. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Long Name	Cluster of Differentiation 45
Entrez Gene IDs	5788 (Human); 19264 (Mouse); 24699 (Rat)
Alternate Names	B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C

Citations for Mouse B220/CD45R PE-conjugated Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse B220/CD45R PE-conjugated Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Al Ghamdi A et al. (2015-01-01), Oral supplementation of diabetic mice with propolis restores the proliferation capacity and chemotaxis of B and T lymphocytes towards CCL21 and CXCL12 by modulating the lipid profile, the pro-inflammatory cytokine levels and oxidative stress. BMC Immunol, 16(0):54.
PMID: 26370805

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