Mouse CD3 Biotinylated Antibody

Catalog # BAM4841 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # 17A2

Product Summary for Mouse CD3 Biotinylated Antibody

Immunogen

T cell hybridoma D1

Specificity

Detects mouse CD3. Recognizes mouse T cell receptor-associated CD3 complex that is expressed on thymocytes and mature T cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CD3 Biotinylated Antibody

Detection of CD3 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD3 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated CD3 Monoclonal Antibody (Catalog # BAM4841) or (B) Rat IgG2B Biotinylated isotype control antibody (Catalog # IC013B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). View our protocol for Staining Membrane-associated Proteins.
CD3 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD3 in Mouse Splenocytes.

CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Biotinylated Monoclonal Antibody (Catalog # BAM4841) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse CD3 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunohistochemistry

Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

Immunoprecipitation

Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAM4841 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References

  1. Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

Alternate Names

CD3, CD3E, CD_antigen: CD3e, CD3 antigen, delta subunit, CD3-DELTA, CD3-epsilon, CD3-GAMMA, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3G, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T3DOKT3, delta chain, T3E, TCRE

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Gene Symbol

CD3E

Product Documents for Mouse CD3 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD3 Biotinylated Antibody

For research use only

Customer Reviews for Mouse CD3 Biotinylated Antibody (1)

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  • Mouse CD3 Biotinylated Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Splenocytes
    Species: Mouse
    Verified Customer | Posted 09/16/2022
    Mouse CD3 Biotinylated Antibody BAM4841

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FAQs for Mouse CD3 Biotinylated Antibody

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  • Q: What is the optimal working concentration of the antibody?

    A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.

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