Mouse splenocytes were stained with Rat Anti-Mouse CD229/SLAMF3 Monoclonal Antibody (Catalog # MAB2555, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B).

Applications for Mouse CD229/SLAMF3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Mouse splenocytes

Western Blot

1 µg/mL
Sample: Recombinant Mouse CD229/SLAMF3 under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD229/SLAMF3

CD229, also known as T lymphocyte surface antigen Ly-9, is a type I transmembrane protein belonging to the immunoglobulin superfamily. It is also a member of the CD150/SLAM receptor family and is expressed on T and B lymphocytes. Mouse CD229 contains 2 Ig-like C2-type domains and 2 Ig-like V-type domains in its extracellular region. Two mouse alleles that differ in 6 extracellular amino acid residues have been reported. Human and mouse CD229 share 60% amino acid sequence identity in their extracellular regions.

Long Name

SLAM Family Member 3

Alternate Names

CD229, Ly9, SLAMF3

Entrez Gene IDs

4063 (Human); 17085 (Mouse)

Gene Symbol

LY9

UniProt

AAH95921

Additional CD229/SLAMF3 Products

Product Documents for Mouse CD229/SLAMF3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD229/SLAMF3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CD229/SLAMF3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2555

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CD229/SLAMF3 Antibody

Detection of CD229/SLAMF3 in Mouse Splenocytes by Flow Cytometry.