Mouse Alkaline Phosphatase/ALPL Antibody

Alkaline Phosphatase/ALPL in Mouse Embryo.

Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2910) at 2 µg/mL overnight at 4 °C. Tissue was stained red and counterstained with DAPI (blue).Image courtesy of Paul J. Simmons, Ph.D., Director, Center for Stem Cell Research, The University of Texas Health Science Center at Houston, Houston, Texas, USA.View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Alkaline Phosphatase/ALPL by Western Blot

Downregulation of HDAC9 rescued lineage differentiation imbalance and ameliorated senescence in aged BMMSCs. a Alizarin Red staining was performed, and osteogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. b Oil Red O staining was performed, and adipogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. c Alizarin Red staining was performed, and osteogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. d Oil Red O staining was performed, and adipogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. e, f Expressions of the senescence-related proteins p53 and p-p53 in BMMSCs cultured in vitro from aged mice (e) and young mice (f) were examined by western blotting. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, one-way analysis of variance (ANOVA) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Alkaline Phosphatase/ALPL by Western Blot

Inhibition of HDAC9 improved the lineage differentiation of endogenous BMMSCs ex vivo. The BMMSCs harvested from the mice from the control, shScr-treated, & shHDAC9-treated groups 4 weeks after bone intrainjection. e Alizarin Red staining was performed, & osteogenesis-related proteins detected in BMMSCs from the three group. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Alkaline Phosphatase/ALPL by Western Blot

The representative images of Ki‐67 staining (A) and quantified by the positive‐stained percentage through ImageJ software (B). Scale bar, 100 μm. (C‐D) Alizarin Red staining was performed to detect mineralized nodules formed in Con, AS‐Exo and NS‐Exo 14 days after osteogenic induction and quantified with a spectrophotometer after dissolving with cetylpyridinium chloride. (E‐F) Lipid droplet formation was detected by Oil Red O staining 7 days after adipogenic induction with positive area quantified. Scale bar, 100 μm. (G‐H) The protein expression levels of Runx2, ALP and PPAR‐ gamma in Con, AS‐Exo and NS‐Exo groups were measured through Western blot and quantified by ImageJ software. n = 3 per group. Data are shown as mean ± SD; ns, not significant; *P < .05; **P < .01; ***P < .001. AS‐Exo, adult serum exosomes; Con, control; NS‐Exo, neonatal serum exosomes Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32608556), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Alkaline Phosphatase/ALPL by Western Blot

Inhibition of autophagy blocked the ability of HDAC9 siRNA to rebalance BMMSC differentiation. To investigate the HDAC9-autophagy axis regulating BMMSC function, BMMSCs were respectively transfected with Nc siRNA and HDAC9 siRNA or co-transfected with HDAC9 siRNA and BECN1 siRNA. a Alizarin Red staining was performed, and osteogenesis-related proteins were analyzed in aged BMMSCs from above three groups. b Oil Red O staining was performed, and adipogenesis-related proteins were analyzed in three groups of cells described above. Scale bars = 100 μm. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, one-way analysis of variance (ANOVA) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.