MMR/CD206/Mannose Receptor Antibody (15-2)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1415

Clone 15-2 was used by HLDA to establish CD designation.
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NB600-1415
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Functional

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 15-2

Concentration

0.1 mg/ml

View all MMR/CD206 Antibodies »

Product Specifications

Immunogen

Purified human mannose receptor from human placental tissue

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MMR/CD206/Mannose Receptor Antibody (15-2)

Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415]

Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415]

Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415] - Frozen sections of human tonsil. NB600-1415 was used ina dilution of 1/25 and stains endothelia of lymph vesselsstrongly.
Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415]

Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415]

Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415] - staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 70% Ethanol and blocking for 10 minutes at RT was performed. The sample was incubated with primary antibody (1/10) for 1 hour. A HRP-conjugated mouse polyclonal to mouse IgG was used undiluted as secondary antibody.

Applications for MMR/CD206/Mannose Receptor Antibody (15-2)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:100

Western Blot

1:10-1:100
Application Notes
For IHC-F: Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum. For FC: Antibody 15-2 stains the extracellular domain of MR. WB: A non-reduced sample treatment and 6% SDS-Page was used. The band size is 175 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MMR/CD206

The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody 15-2 prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Entrez Gene IDs

4360 (Human)

Gene Symbol

MRC1

UniProt

P22897

Additional MMR/CD206 Products

Product Documents for MMR/CD206/Mannose Receptor Antibody (15-2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MMR/CD206/Mannose Receptor Antibody (15-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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