IL-18/IL-1F4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81607

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NBP1-81607-25ul
NBP1-81607
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IL-18/IL-1F4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IL-18/IL-1F4 Antibody

IL-18/IL-1F4 Antibody Immunohistochemistry-Paraffin: IL-18/IL-1F4 Antibody [NBP1-81607]

Immunohistochemistry-Paraffin: IL-18/IL-1F4 Antibody [NBP1-81607]

Analysis in human lymph node and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
IL-18/IL-1F4 Antibody Immunohistochemistry-Paraffin: IL-18/IL-1F4 Antibody [NBP1-81607]

Immunohistochemistry-Paraffin: IL-18/IL-1F4 Antibody [NBP1-81607]

Staining of human spleen shows moderate cytoplasmic positivity.
IL-18/IL-1F4 Antibody Immunohistochemistry-Paraffin: IL-18/IL-1F4 Antibody [NBP1-81607]

Immunohistochemistry-Paraffin: IL-18/IL-1F4 Antibody [NBP1-81607]

Staining of human lymph node shows positivity in lymphoid cells.
View 5 more images

Applications for IL-18/IL-1F4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

-2146826246

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-18/IL-1F4

The protein encoded by the IL18 gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. (provided by RefSeq)

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Gene Symbol

IL18

Additional IL-18/IL-1F4 Products

Product Documents for IL-18/IL-1F4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-18/IL-1F4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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