Human ULBP-2/5/6 PE-conjugated Antibody

Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry.

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 PE-conjugated Monoclonal Antibody (Catalog # FAB1298P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of ULBP-2/5/6 by Flow Cytometry

Soluble ULBP2 does not promote tumor growth. (A) Flow cytometric evaluation of GFP expression in B16BL6-ULBP2 cells. Black and gray histograms represent unstained B16BL6-ULBP2 cells and unstained wild-type B16BL6 cells, respectively. (B) Flow cytometric evaluation of ULBP2 expression in B16BL6-ULBP2 cells. Black and gray histograms represent cells stained with PE-conjugated anti-ULBP2/5/6 antibody and PE-conjugated isotype control antibody, respectively. (C) Measurement of soluble ULBP2 concentrations in the culture supernatants of B16BL6-ULBP2 cells. Cells (1.0 × 104 or 5.0 × 104) were seeded in 24-well plates and cultured. Supernatants were collected at 24 and 48 h, and soluble ULBP2 levels were quantified using ELISA (n = 3). (D) Cell proliferation curves of B16BL6-ULBP2 cells seeded in 6-well plates (5.0 × 104 cells/well). Cells were harvested and counted every 24 h for 5 days using a hemocytometer (n = 6). (E) Specific growth rates (μ) and doubling times (Td) of cells calculated as described in (D). (F) Tumor growth curves of syngeneic subcutaneous tumors in C57BL/6 mice (n = 5) injected with B16BL6-mock or B16BL6-ULBP2 cells (1.0 × 106). Tumor volumes were calculated as described in (E). (G) Tumor weights measured on day 14 for the mice in (F). (H) Soluble ULBP2 concentrations in plasma collected on day 14, measured using ELISA from the mice in (F). In (C–F), data are presented as the mean ± SEM. ** p < 0.01; **** p < 0.0001; ns: not significant (two-tailed Welch’s t-test for C; Mann–Whitney U test for (D–F)). In (G,H), individual values are shown with the mean ± SEM. ** p < 0.01; ns: not significant (Mann–Whitney U test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40243581), licensed under a CC-BY license. Not internally tested by R&D Systems.